[Histonet] Ethanol in tissue processing

Sun May 13 12:04:51 CDT 2018

Thank you all for your answers, our RG alcohol is 3% methanol. You are 
confirming what I thought, we are using the same type of alcohol as most 
histology labs.

Jamie

On May 13, 2018 7:37:52 AM "Jacox, Robert A. via Histonet" 
<histonet at lists.utsouthwestern.edu> wrote:

> Jay,
>
> Michael's post is correct and very well stated. In the US we estimate 
> between 80 -85% of labs now use and RGA.  The most common blend in the U.S 
> is 95-5 which is 95% ethanol denatured with 5% methanol. Any company you 
> purchase from should be happy to provide a C of A (certificate of analysis) 
> to verify how they denature.
>
> Hope this helps.
>
> Robert Jacox
> Product Manager
> Thermo Fisher Scientific
>
> Sent from my iPad
>
>> On May 12, 2018, at 5:21 PM, Michael Gudo (Morphisto GmbH) via Histonet 
>> <histonet at lists.utsouthwestern.edu> wrote:
>>
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>>
>> Well, in Germany, and as far as I know in Europe, most labs use ethanol at 
>> 96 and 99 percent as the last two steps in tissue processing. These 
>> ethanols are are denatured with MEK (= Butanon, Methylethylketon). Methanol 
>> or isopropyl is not allowed as denaturation agent in Europe, otherwise you 
>> have to pay the full alcohol tax.
>>
>> In our lab we we use 96 % ethanol and as last step and then,  before going 
>> to xylene we use isopropanol which has a better dehydration effect than 
>> ethanol. 99 % ethanol takes water from the air and quite fast becomes a 96 
>> or 95 % ethanol and you will have some water left in the specimens when you 
>> to to xylene.
>> So our experience are that the best quality of dehydration can be reached 
>> with ethanol as high as possible and finally isopropanol before the xylene 
>> (2 steps).
>>
>> With best regards
>> Michael
>>
>>
>>> Am 12.05.2018 um 22:48 schrieb Jay Lundgren via Histonet 
>>> <histonet at lists.utsouthwestern.edu>:
>>>
>>> Most places use "reagent alcohol" which, from most suppliers, is a mongrel
>>> blend of whatever is cheapest, and not even listed on the label. It's
>>> usually made up of a blend of ethanol, methanol, and isopropyl.  Good luck
>>> finding what the percentages are.  I've called one vendor before, and even
>>> they couldn't tell me.
>>>
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>>> On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet <
>>> histonet at lists.utsouthwestern.edu> wrote:
>>>
>>>> We use reagent alcohol on the processor and for staining.
>>>>
>>>> Get Outlook for 
>>>> iOS<https://urldefense.proofpoint.com/v2/url?u=https-3A__aka.ms_o0ukef&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=G68TvqrXQ5gMkZ7yXrFjlTfSBXBWHSyCXohNpKIZAgI&e=>
>>>> ________________________________
>>>> From: Jamie Watson via Histonet <histonet at lists.utsouthwestern.edu>
>>>> Sent: Saturday, May 12, 2018 9:06:01 AM
>>>> To: Histonet at lists.utsouthwestern.edu
>>>> Subject: [Histonet] Ethanol in tissue processing
>>>>
>>>> Hi All,
>>>>
>>>> Does anyone know what percentage of labs use ethanol vs. denatured alcohol
>>>> or isopropyl alcohol on the tissue processor?
>>>>
>>>> Jamie
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