[Histonet] Ethanol in tissue processing

Sun May 13 09:29:47 CDT 2018

Jay,

Michael's post is correct and very well stated. In the US we estimate between 80 -85% of labs now use and RGA.  The most common blend in the U.S is 95-5 which is 95% ethanol denatured with 5% methanol. Any company you purchase from should be happy to provide a C of A (certificate of analysis) to verify how they denature. 

Hope this helps.

Robert Jacox
Product Manager
Thermo Fisher Scientific

Sent from my iPad

> On May 12, 2018, at 5:21 PM, Michael Gudo (Morphisto GmbH) via Histonet <histonet at lists.utsouthwestern.edu> wrote:
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> Well, in Germany, and as far as I know in Europe, most labs use ethanol at 96 and 99 percent as the last two steps in tissue processing. These ethanols are are denatured with MEK (= Butanon, Methylethylketon). Methanol or isopropyl is not allowed as denaturation agent in Europe, otherwise you have to pay the full alcohol tax.
> 
> In our lab we we use 96 % ethanol and as last step and then,  before going to xylene we use isopropanol which has a better dehydration effect than ethanol. 99 % ethanol takes water from the air and quite fast becomes a 96 or 95 % ethanol and you will have some water left in the specimens when you to to xylene.
> So our experience are that the best quality of dehydration can be reached with ethanol as high as possible and finally isopropanol before the xylene (2 steps).
> 
> With best regards
> Michael
> 
> 
>> Am 12.05.2018 um 22:48 schrieb Jay Lundgren via Histonet <histonet at lists.utsouthwestern.edu>:
>> 
>> Most places use "reagent alcohol" which, from most suppliers, is a mongrel
>> blend of whatever is cheapest, and not even listed on the label. It's
>> usually made up of a blend of ethanol, methanol, and isopropyl.  Good luck
>> finding what the percentages are.  I've called one vendor before, and even
>> they couldn't tell me.
>> 
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>> On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet <
>> histonet at lists.utsouthwestern.edu> wrote:
>> 
>>> We use reagent alcohol on the processor and for staining.
>>> 
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>>> ________________________________
>>> From: Jamie Watson via Histonet <histonet at lists.utsouthwestern.edu>
>>> Sent: Saturday, May 12, 2018 9:06:01 AM
>>> To: Histonet at lists.utsouthwestern.edu
>>> Subject: [Histonet] Ethanol in tissue processing
>>> 
>>> Hi All,
>>> 
>>> Does anyone know what percentage of labs use ethanol vs. denatured alcohol
>>> or isopropyl alcohol on the tissue processor?
>>> 
>>> Jamie
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