[Histonet] Histonet Digest, Vol 174, Issue 9

[Bob Richmond]

Jamie Watson, you need to clarify this question: >> Does anyone know what
percentage of labs use ethanol vs. denatured alcohol or isopropyl alcohol
on the tissue processor?<<

100% ethanol is perfectly OK, but it requires a lot of regulator compliance
because you can drink it (though you shouldn't).

The usual denatured alcohol for histology is customarily called "reagent
alcohol". It contains 90% ethanol, 5% methanol, and 5% isopropanol. The
BATF calls it "S3D modified". It's also possible to denature with methyl
isobutyl ketone (MIBK) but it smells horrible. Alcohol denatured with
acetone is available, but it'll extract your eosin - you need to know about
it because an occasional manager will insist on it.

All of these are equivalent from a practical viewpoint. Isopropyl alcohol
(isopropanol) is of course quite different, and I have no idea how many
labs use it.

Bob Richmond
Samurai Pathologist
Maryville TN

On Sat, May 12, 2018 at 1:00 PM, <histonet-request at lists.utsouthwestern.edu>
wrote:

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> Date: Sat, 12 May 2018 16:06:01 +0000
> From: Jamie Watson <jamie at watson-home.com>
> To: "Histonet at lists.utsouthwestern.edu"
>         <Histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Ethanol in tissue processing
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> Hi All,
>
> Does anyone know what percentage of labs use ethanol vs. denatured alcohol
> or isopropyl alcohol on the tissue processor?
>
> Jamie
>
>
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> End of Histonet Digest, Vol 174, Issue 9
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