[Histonet] Slide labels falling off- Slidemate AS

[Mike Toole]

Stefanie,

>> the labels peel off and float away during staining

Sounds like a paper type label or more likely a xylene resistant type label. Either way, try applying the label after the slides are stained instead of before staining.

Mike

-----Original Message-----
From: Laurie Colbert via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, March 28, 2018 11:02 AM
To: Stefanie.Harris at crl.com; histonet at lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Slide labels falling off- Slidemate AS

Stefanie,

When you say "labels" are  you talking about paper labels or the print on the slides from the Slidemate??

I will assume that you mean the print, and yes, I have had the problem in the past where the print came off during staining and/or you could literally wipe the print off with a kimwipe or your finger.  In our case, it was the slides - even though it was the same slide we had been using for years.  This was happening with a certain lot number.  Is this happening with just one lot number?  Maybe you can try and get a different lot number or try a totally different slide (different manufacturer) to narrow down the issue.
Good luck!
Laurie



-----Original Message-----
From: Harris, Stefanie via Histonet <histonet at lists.utsouthwestern.edu>
To: histonet <histonet at lists.utsouthwestern.edu>
Sent: Wed, Mar 28, 2018 8:51 am
Subject: [Histonet] Slide labels falling off- Slidemate AS

Hello all,

I am hoping someone out there can help. We have been having issues with some of the labels coming off during staining and after. It seems to be random so I can't tell if there is a problem with the slides or the Slidemate AS. There are days that it will be half the slides but other days where it will be none. Sometimes the labels peel off and float away during staining and other times labels rub off when handling but only after staining.


We are using Globe Scientific Slides, and have been for over 2 years with no issues until December.

I have changed out the ribbon on the slidemate and the problem is still sporadic.

Oven time drying time does not seem to be a factor.

We have not made any reagent changes for the staining process to explain the issue either.


Has anyone else seen anything like this? Any slide suggestions to try out?

Stefanie Harris
Research Assistant, Histology
Research Animal Diagnostic Services | Charles River
261 Ballardvale St., Wilmington, MA 01887
P: 781.222.6592 | F: 978.658.7698
stefanie.harris at crl.com | www.criver.com<http://www.criver.com/>
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