[Histonet] IHC validation

Morken, Timothy Timothy.Morken at ucsf.edu
Thu Mar 22 11:12:18 CDT 2018

I've used hundreds of TMA's from Pantomics and Biochain with great results. 



Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-----Original Message-----
From: Dessoye, Michael via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, March 22, 2018 9:00 AM
To: Terri Braud; 'histonet at lists.utsouthwestern.edu'
Subject: Re: [Histonet] IHC validation

Can anyone recommend a vendor that they've had good luck with for TMA slides?

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484

-----Original Message-----
From: Terri Braud [mailto:tbraud at holyredeemer.com] 
Sent: Tuesday, March 20, 2018 1:54 PM
To: 'histonet at lists.utsouthwestern.edu'
Subject: Re: [Histonet] IHC validation

Just another note:  You can order unstained tissue microarrays with the prerequisite number of cases, both positive and negative, and stain your validation all on one slide.  I've done this for years and for 3 different validations of entire IHC platform changes, ranging from 40 to over 100 antibodies each time.  Saves time and money.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Message: 2
Date: Fri, 16 Mar 2018 06:54:30 -0700
From: "Paula" <plucas at biopath.org>
Subject: [Histonet] Antibody Validation CLIA

We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation.  I think CAP requires 20 slides..?
And so we are asking if there is  a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification.
Thank you in advance

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