[Histonet] IHC validation

Ana Maluenda Ana.Maluenda at baker.edu.au
Thu Mar 22 01:30:00 CDT 2018

Hi Terri,

This is actually a very valid note. This has been in my mind for years, but never had the chance to put in action in a research environment (I always try to bring to the research field the efficiency and money saving strategies we use in diagnostics).

Where do you usually order your unstained slides from?

Much appreciated for the advice.

Kind regards,


Ana Maluenda
Research Assistant
Atherothrombosis and Vascular Biology Laboratory

Baker Heart and Diabetes Institute
75 Commercial Road, Melbourne VIC 3004
P (03) 8532 1359 E Ana.Maluenda at baker.edu.au W www.baker.edu.au

-----Original Message-----
From: Terri Braud [mailto:tbraud at holyredeemer.com]
Sent: Wednesday, 21 March 2018 4:54 AM
To: 'histonet at lists.utsouthwestern.edu' <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] IHC validation

Just another note:  You can order unstained tissue microarrays with the prerequisite number of cases, both positive and negative, and stain your validation all on one slide.  I've done this for years and for 3 different validations of entire IHC platform changes, ranging from 40 to over 100 antibodies each time.  Saves time and money.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Message: 2
Date: Fri, 16 Mar 2018 06:54:30 -0700
From: "Paula" <plucas at biopath.org>
Subject: [Histonet] Antibody Validation CLIA

We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation.  I think CAP requires 20 slides..?
And so we are asking if there is  a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification.
Thank you in advance

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