[Histonet] IHC validation

Colleen Forster cforster at umn.edu
Tue Mar 20 13:27:19 CDT 2018

I agree Terry,

The TMA slide is a very economical and powerful way to validate with
minimal slides needed.

Colleen Forster

On Tue, Mar 20, 2018 at 12:54 PM, Terri Braud via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Just another note:  You can order unstained tissue microarrays with the
> prerequisite number of cases, both positive and negative, and stain your
> validation all on one slide.  I've done this for years and for 3 different
> validations of entire IHC platform changes, ranging from 40 to over 100
> antibodies each time.  Saves time and money.
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
> Message: 2
> Date: Fri, 16 Mar 2018 06:54:30 -0700
> From: "Paula" <plucas at biopath.org>
> Subject: [Histonet] Antibody Validation CLIA
> Hello,
> We've been discussing about the quantity of slides to run as a validation
> for IHC antibodies. We are governed by CLIA, and we would like to know if
> there is a set number of slides to run for a particular antibody we would
> like to bring in-house for Validation.  I think CAP requires 20 slides..?
> And so we are asking if there is  a requirement with CLIA to run a certain
> number of slides, or is it up to us (the laboratory director) to decide how
> many slides to run for Validation/Verification.
> Thank you in advance
> Paula
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