[Histonet] Histonet Digest, Vol 172, Issue 7
Hayden, Christopher
christopher.hayden at novartis.com
Mon Mar 12 07:22:00 CDT 2018
Good Day, Tim:
I'll second what Terri said. My first concern with embedding a contact lens would be that the embedding media and/or transitional solvents would attack the lens and/and dislodge the bacteria. Unless you have a few lenses to do a method development pilot study with, I'd shy away from a full embedding. If you had to go the TEM route, maybe a plastic like LR White, as the dehydration doesn't have to be as aggressive.
The SEM is probably the best tool for this sort of investigation; we've had luck with identifying bacterial strains on morphology alone with our SEM, or at least it got us into the ballpark enough for the investigation.
Children's Colorado has an SEM in their facility.
Cheers,
-Chris
Christopher Hayden
Electron Microscopy / Digital Imaging
LS - Specialty Laboratories
T +1 862 778 7993
christopher.hayden at novartis.com
Novartis Institutes for Biomedical Research
Date: Fri, 9 Mar 2018 19:30:27 +0000
From: "Terri Braud" <tbraud at holyredeemer.com>
To: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Identification of bacteria on an acrylic lens
prosthesis
Message-ID:
<48E053DDF6CE074DB6A7414BA05403F8B2325A46 at HRHEX02-HOS.holyredeemer.local>
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I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The scanning electron microscope (SEM) can also be useful to reveal morphological features of isolated organisms as well as for diagnosis. There are numerous articles citing this technique. I believe the UC Berkley has an SEM in their lab. I hope this helps. Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Today's Topics:
1. Prosthetic lens processing, cutting? (Morken, Timothy)
Message: 1
Date: Thu, 8 Mar 2018 19:30:35 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Prosthetic lens processing, cutting?
Hi all,
Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it.
Any ideas?
Any labs that handle this kind of thing? They are willing to send it out.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center
Christopher Hayden
T +1 862 778 7993
christopher.hayden at novartis.com
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Subject: Histonet Digest, Vol 172, Issue 7
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Today's Topics:
1. Re: Histonet Digest, Vol 172, Issue 6 "Slide labels or slide
Printer" (Brent Adams)
2. Re: Identification of bacteria on an acrylic lens prosthesis
(Terri Braud)
3. SAVE THE DATE! HISTOPALOOZA 2018 APRIL 20-APRIL 22
(Zimmerman, Billie)
4. What is the smallest diameter (easily available and cheap)
syringe? (Jorge A. Santiago-Blay)
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Message: 1
Date: Fri, 9 Mar 2018 18:48:14 +0000
From: Brent Adams <badams at acadianagastro.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Histonet Digest, Vol 172, Issue 6 "Slide
labels or slide Printer"
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I also have used Sakura Smart Write (Primera printers repackaged under the Sakura name) slide and cassette printers since they came out. I had some issues with the slide printers in the beginning but they have worked out all the bugs and greatly improved the machine. I do very little maintenance other than clean rollers on the slide printer and never had any issues with the cassette printer. I recommend them for Small Labs for sure. They have multi cassette holders for larger labs.
Hope that helps
Brent Adams ? BS, LPN, HT
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Today's Topics:
1. Prosthetic lens processing, cutting? (Morken, Timothy)
2. Re: Slide labels or slide labeler (Blazek, Linda)
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Message: 1
Date: Thu, 8 Mar 2018 19:30:35 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Prosthetic lens processing, cutting?
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Hi all,
Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it.
Any ideas?
Any labs that handle this kind of thing? They are willing to send it out.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center
------------------------------
Message: 2
Date: Thu, 8 Mar 2018 15:32:57 -0500
From: "Blazek, Linda" <lblazek at digestivespecialists.com>
To: Victor <victor_tobias at comcast.net>, "Vickroy, James"
<jvickroy at SpringfieldClinic.com>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Slide labels or slide labeler
Message-ID:
<5A2BD13465E061429D6455C8D6B40E392A826E8032 at IBMB7Exchange.digestivespecialists.com>
Content-Type: text/plain; charset="utf-8"
I've used the Primera slide printer since it came out. I like it a lot. It needs it's maintenance kept up like anything else but other than cleaning the rollers there is very little else that needs cleaning.
Linda Blazek HT (ASCP)
Pathology Lab Manager
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lblazek at digestivespecialists.com
-----Original Message-----
From: Victor via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, March 07, 2018 5:59 PM
To: Vickroy, James; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Slide labels or slide labeler
James,
I totally agree with your assessment of the Slidemate printers. Have not had any experience with the newer model.
Applying slide labels shouldn?t be an issue when dealing with one block and slide(s) at a time. Some of this could depend upon your software available to you. Our system generated just the labels needed for the block you were cutting, on demand. You should be able to get a nice Zebra printer for under $700.
Another of our labs used the new Sakura/Premera printer and really like it.
Victor
Sent from Mail for Windows 10
From: Vickroy, James via Histonet
Sent: Wednesday, March 7, 2018 2:12 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Slide labels or slide labeler
We have used the older version Slidemate slide labelers from Thermofisher for the last three years. I think putting the patient information directly on the slide is preferred however the wear and tear on slide printers means higher maintenance costs and more down time. Thermofisher has a newer version of the Slidemate now that has been designed so that there is less wear and tear on the machines. Currently when one of the old labelers breaks down we have to send the instrument out and the cost is generally 1000 - 1500 for repair each time. It also seems that the older machines have to be sent in at least every other year. Our older machines have been sent in almost yearly.
We are considering switching to a slide label making system by General Data. One of the downsides is that we have to apply the label to each slide so therefore we introduce another step where the slides could be mislabeled. The upside is that equipment costs are much less. A new thermal label printer runs around 700.00 where as the instrument that imprints the slide label on the slide runs between 12000 - 15000 per unit. We need three units.
Thermo also offers a lease system. What I need to know is what has been the experience with the new Slidemate AS printers? What kind of maintenance issues have you encountered? Finally please let me know your latest experiences with both slide label printers versus slide labeling printers?
Thanks for your input.
Jim
Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois 62703
Office: 217-528-7541, Ext. 15121
Email: jvickroy at SpringfieldClinic.com<mailto:jvickroy at SpringfieldClinic.com>
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Message: 2
Date: Fri, 9 Mar 2018 19:30:27 +0000
From: "Terri Braud" <tbraud at holyredeemer.com>
To: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Identification of bacteria on an acrylic lens
prosthesis
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I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The scanning electron microscope (SEM) can also be useful to reveal morphological features of isolated organisms as well as for diagnosis. There are numerous articles citing this technique. I believe the UC Berkley has an SEM in their lab. I hope this helps. Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
Today's Topics:
1. Prosthetic lens processing, cutting? (Morken, Timothy)
Message: 1
Date: Thu, 8 Mar 2018 19:30:35 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Prosthetic lens processing, cutting?
Hi all,
Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it.
Any ideas?
Any labs that handle this kind of thing? They are willing to send it out.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center
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Message: 3
Date: Fri, 9 Mar 2018 19:43:06 +0000
From: "Zimmerman, Billie" <BZIMMERM at augusta.edu>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] SAVE THE DATE! HISTOPALOOZA 2018 APRIL
20-APRIL 22
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<BN6PR03MB25946D3A4DC67341A904F832CBDE0 at BN6PR03MB2594.namprd03.prod.outlook.com>
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TGIF everyone
The Georgia Society for Histotechnology meeting will be at the Callaway Gardens Lodge, Pine Mountain, GA More details to follow, but for now save the date!
Billie Zimmerman
------------------------------
Message: 4
Date: Sat, 10 Mar 2018 10:43:45 -0500
From: "Jorge A. Santiago-Blay" <blayjorge at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] What is the smallest diameter (easily available
and cheap) syringe?
Message-ID:
<CAGBdDuArmduaFBYTDLe-35ErFLYzSycud7j1WVXuGzPoct6bcw at mail.gmail.com>
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Hi:
I am preparing a device for blowing air into a tiny, subcylindrical structure (diameter << 1 mm) in some (non-human) organisms. Heat extruded Pasteur pipettes are good but too fragile for my taste.
What is the smallest diameter (easily available and cheap) syringe?
If you have any constructive suggestions, please email me directly.
Cheers,
Jorge
Jorge A. Santiago-Blay, PhD
blaypublishers.com
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