[Histonet] Histonet Digest, Vol 175, Issue 15

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Mon Jun 18 09:06:35 CDT 2018

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From: Nava, Josefa via Histonet
To: histonet at lists.utsouthwestern.edu
Sent: June 18, 2018 at 4:25 AM
Subject: Re: [Histonet] Histonet Digest, Vol 175, Issue 15

unsubscribe -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, June 16, 2018 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 175, Issue 15 [EXTERNAL] Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest.
.." Today's Topics: 1. Staining Protocol for Chromosome Counting in Plants (Bharti Parihar) ---------------------------------------------------------------------- Message: 1 Date: Fri, 15 Jun 2018 15:09:00 -0700 From: Bharti Parihar To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Staining Protocol for Chromosome Counting in Plants Message-ID: Content-Type: text/plain; charset="UTF-8" Hi ya'll. If there are any individuals out there that have done karyotyping, I need to try a protocol to get a chromosome count on a desert plant. We have germinated seeds with long roots, so sample size shouldn't be an issue. Tried a Orcein stain, but the results are quite lacking and it may not be theright stain for this plant. Gonna do a bit of modification with the fixative to specimen ratio to see if that helps, but it's possible this just isn't the right stain. After all, the stain ordered is for Arabidopsis, which is not our research plant. This is my first time doing plant histo, I'm us
ed to human tissue, so I really don't know which stain to try. Anyone with any help/advice to offer, I would immensely appreciate it! -- Bharti Parihar, HT (ASCP)CM ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= ------------------------------ End of Histonet Digest, Vol 175, Issue 15 ***************************************** The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If yo
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