[Histonet] Histonet Digest, Vol 175, Issue 15

Nava, Josefa JosefaNava at texashealth.org
Mon Jun 18 06:23:03 CDT 2018


unsubscribe

-----Original Message-----
From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] 
Sent: Saturday, June 16, 2018 12:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 175, Issue 15

[EXTERNAL]

Send Histonet mailing list submissions to
	histonet at lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e=
or, via email, send a message with subject or body 'help' to
	histonet-request at lists.utsouthwestern.edu

You can reach the person managing the list at
	histonet-owner at lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Staining Protocol for Chromosome Counting in Plants
      (Bharti Parihar)


----------------------------------------------------------------------

Message: 1
Date: Fri, 15 Jun 2018 15:09:00 -0700
From: Bharti Parihar <bhartologist at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Staining Protocol for Chromosome Counting in
	Plants
Message-ID:
	<CAJxUEriJH7rNRJ7pPPEP=8mjxk8pck6ZxuO=8j2w11yHf_nzqA at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"

Hi ya'll. If there are any individuals out there that have done karyotyping, I need to try a protocol to get a chromosome count on a desert plant. We have germinated seeds with long roots, so sample size shouldn't be an issue. Tried a Orcein stain, but the results are quite lacking and it may not be the right stain for this plant. Gonna do a bit of modification with the fixative to specimen ratio to see if that helps, but it's possible this just isn't the right stain. After all, the stain ordered is for Arabidopsis, which is not our research plant. This is my first time doing plant histo, I'm used to human tissue, so I really don't know which stain to try. Anyone with any help/advice to offer, I would immensely appreciate it!

--
Bharti Parihar, HT (ASCP)CM


------------------------------

Subject: Digest Footer

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e=

------------------------------

End of Histonet Digest, Vol 175, Issue 15
*****************************************

The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system.



More information about the Histonet mailing list