[Histonet] Fat Aspirates for Amyloidosis

[Lindrud, Scott]

Hi Histonetters,

Would anyone be willing to share how they process a Fat Pad aspirate for Amyloidosis?  Our method does not provide a very good result and we're looking to improve on how we do this.

We collect direct aspirates using an 18 gauge needle and make air dried smears and stain with Congo Red.  We also get additional passes and place directly in 10% NBF and will try to make a cell block using histogel out of them.  Problem is fat floats and it is difficult to get a good cell block made.

I would think spinning the 10%NBF and filtering out the fat and placing directly into a biopsy bag or wrapping in paper would be the way to go.  After you process the block, I'm guessing all the lipid will process out and there would only be cell membranes from the fibroadipose tissue left over.  Wouldn't you need a significant amount of fat to process to have anything to embed?  I've seen suggestions on other websites suggesting 35-50 mg of fat.  Maybe we need to go to a larger needle, maybe 16 gauge?

Any ideas would be greatly appreciated!

Scott



Scott A. Lindrud, MLS(ASCP) CM CT CM
Histopathology Technical Specialist/Cytotechnologist
CarrisHealth Rice Memorial Hospital
301 Becker Ave SW | Willmar | MN | 56201
scott.lindrud at carrishealth.com
320-231-4406/4520
320-231-4503 (fax)

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