[Histonet] Hazy/cooked cells in H&E

[Cristi Rigazio]

Have you checked the processors?  It is easy to “mix up” the reagents.  
Has tissue collection changed? Are the specimens left out longer or placed on paper? 
 Do you use sponges?  Any chance the fixation time has been decreased?

Sent from my iPhone

> On Jul 18, 2018, at 3:46 PM, Mac Donald, Jennifer via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Is anyone using freezing spray?  We had this problem and all cases were traced back to the same microtomist. She used the freezing spray excessively and was causing “freezer burn” to the GI biopsies.
> Jennifer
> 
> Get Outlook for iOS<https://aka.ms/o0ukef>
> 
> ________________________________
> From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Wednesday, July 18, 2018 11:12 AM
> To: Histo List
> Subject: [Histonet] Hazy/cooked cells in H&E
> 
> Our pathologists have said that we have had a few cases the past few days
> that are unreadable. They say the cells appear "cooked".
> 
> Nothing in our process has changed, we have been using the same lot of
> reagents since the middle of June, and no errors were reported in our
> processing runs.
> 
> Do anyone have any ideas to what could be causing this issue?
> 
> 
> --
> 
> Charles Riley BS HT, HTL(ASCP)CM
> 
> Histopathology Coordinator/ Mohs
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet