[Histonet] Hazy/cooked cells in H&E

[Mac Donald, Jennifer]

Is anyone using freezing spray?  We had this problem and all cases were traced back to the same microtomist. She used the freezing spray excessively and was causing “freezer burn” to the GI biopsies.
Jennifer

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________________________________
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, July 18, 2018 11:12 AM
To: Histo List
Subject: [Histonet] Hazy/cooked cells in H&E

Our pathologists have said that we have had a few cases the past few days
that are unreadable. They say the cells appear "cooked".

Nothing in our process has changed, we have been using the same lot of
reagents since the middle of June, and no errors were reported in our
processing runs.

Do anyone have any ideas to what could be causing this issue?


--

Charles Riley BS HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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