[Histonet] Subject: Re: Trouble shooting question

[Tim Higgins]

Hey Charles,

I have to agree with Rene.  They are either being left out after sampling prior to fixation or possibly coming into contact with air during the processing run.  Make sure your solution are covering all the cassettes, don't let them float free inside the processing chamber.

Thanks,

Tim
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Message: 6
Date: Thu, 12 Jul 2018 12:26:21 -0400
From: Charles Riley <criley at dpspa.com>
To: Histo List <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Trouble shooting question
Message-ID:
	<CAAQhB10saum8V91Ky9PutieDb55xwAtENj05jFDOPQ7sk2uXTg at mail.gmail.com>
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My pathologists says that 1 out of 100 (his estimate) of our FNA's
cellblocks  apears to be "cooked" when looking at the slides.  The process
has never been changed and all reagents are the same all the time.

 What is/are the possible cause(s) and what can be done to prevent this 1
out of 100 situation?




-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs


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Message: 7
Date: Thu, 12 Jul 2018 16:49:36 +0000 (UTC)
From: Rene J Buesa <rjbuesa at yahoo.com>
To: Histo List <histonet at lists.utsouthwestern.edu>, 	Charles Riley
	<criley at dpspa.com>
Subject: Re: [Histonet] Trouble shooting question
Message-ID: <377951180.2930091.1531414176756 at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

 Check fixation time and specially IF the Bx was left to dry before fixation.Ren?

    On ?Thursday?, ?July? ?12?, ?2018? ?12?:?38?:?28? ?PM, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 My pathologists says that 1 out of 100 (his estimate) of our FNA's
cellblocks? apears to be "cooked" when looking at the slides.? The process
has never been changed and all reagents are the same all the time.

 What is/are the possible cause(s) and what can be done to prevent this 1
out of 100 situation?




-- 

Charles Riley BS? HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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End of Histonet Digest, Vol 176, Issue 7
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