[Histonet] [EXTERNAL] Deplastifying MMA

[Garcia, Lori, M.Sc.]

Hi Reuel,

One thing you could try is THF (tetrahydrofuran). We have used it in the past to rapidly dissolve the MMA on slides.

Good luck,
Lori

-----Original Message-----
From: Dorothy Hu via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, February 14, 2018 11:46 AM
To: histonet at lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] Deplastifying MMA

>
> Hi  Reuel
>

We had the same thing as you. It was caused by bad MMA, I tested. Write down the lot # and ask refund. Test new lot # 1st of MMA in the future. It was very frustrating....I know.
Best luck.

Dorothy





> Message: 2
> Date: Mon, 12 Feb 2018 21:07:15 +0000
> From: Reuel Cornelia <Reuel.Cornelia at tsrh.org>
> To: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Deplastifying MMA
> Message-ID:
>         <SN1PR17MB03668392B3AC2DDACA8FBA92E7F70 at SN1PR17MB0366.
> namprd17.prod.outlook.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello histonetters,
>
> I have a problem with my MMA plastic section on my slide. I could not
> remove the plastic MMA even if I leave the section slides in Xylene
> for several days with heat incubation at 60 degrees. This is the first
> time that happened to my section for several years of doing the same thing over.
> I was thinking that it was my Xylene lot number so I tried to change
> it but still does not remove the plastic, and then Itried different
> solvent Acetone, and Ethylene glycol Monoethyl ether but still it does not work.
> Can I ask for help if anyone knows what was going on and what would be
> the best way to remove this plastic from my section?
>
> Just to give you my embedding solution was MMA -94% , dibutly
> phthalate- 5% and perkadox 16- 0.5%. This have been my solution for
> years and I do not have any problem with the removal of plastic
> section until now. I was thinking that my MMA (M55909) different lot
> number from Sigma Aldrich may have cause this because even I tried to
> use the MMA to dissolve my plastic it does not work.
>
>
> Thank you for and any opinions or protocols are greatly appreciated.
>
>
> Reuel Cornelia
>
> TSRH
>
> 214-559-7766
>
>
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