[Histonet] Deplastifying MMA

[Dorothy Hu]

>
> Hi  Reuel
>

We had the same thing as you. It was caused by bad MMA, I tested. Write
down the lot # and ask refund. Test new lot # 1st of MMA in the future. It
was very frustrating....I know.
Best luck.

Dorothy





> Message: 2
> Date: Mon, 12 Feb 2018 21:07:15 +0000
> From: Reuel Cornelia <Reuel.Cornelia at tsrh.org>
> To: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Deplastifying MMA
> Message-ID:
>         <SN1PR17MB03668392B3AC2DDACA8FBA92E7F70 at SN1PR17MB0366.
> namprd17.prod.outlook.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello histonetters,
>
> I have a problem with my MMA plastic section on my slide. I could not
> remove the plastic MMA even if I leave the section slides in Xylene for
> several days with heat incubation at 60 degrees. This is the first time
> that happened to my section for several years of doing the same thing over.
> I was thinking that it was my Xylene lot number so I tried to change it but
> still does not remove the plastic, and then Itried different solvent
> Acetone, and Ethylene glycol Monoethyl ether but still it does not work.
> Can I ask for help if anyone knows what was going on and what would be the
> best way to remove this plastic from my section?
>
> Just to give you my embedding solution was MMA -94% , dibutly phthalate-
> 5% and perkadox 16- 0.5%. This have been my solution for years and I do not
> have any problem with the removal of plastic section until now. I was
> thinking that my MMA (M55909) different lot number from Sigma Aldrich may
> have cause this because even I tried to use the MMA to dissolve my plastic
> it does not work.
>
>
> Thank you for and any opinions or protocols are greatly appreciated.
>
>
> Reuel Cornelia
>
> TSRH
>
> 214-559-7766
>
>