[Histonet] MMR Negative controls

[Allan Wang]

 Hi,

We (at US Biomax) recently screened our colon TMAs for loss of expression
for MLH1, MSH2, MSH6, PMS2 using Ventana's antibodies.

If you need around 10 cases of negative tissue:
CO20813a, 208 cases of colon, contains loss of:
PMS2: 35
MLH1: 18
MSH6: 14
MSH2: 8

This TMA is not listed on our website but is similar in design to CO20813.
We have additional results on other 208 case TMAs too. Feel free to contact
me directly for more details.

Allan

Allan Wang
Lab Manager
US Biomax, Inc.

On Mon, Feb 12, 2018 at 10:37 AM, Lindrud, Scott via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Good Morning HistoNet,
>
> Would anyone be willing to share what they use for control tissue for MMR
> testing?  Finding negative expressing tumor is becoming more difficult for
> us to find and we're wondering if there is an alternate negative tissue
> that would be acceptable.
>
> NordiQC recommends using Tonsil as a positive tissue control and Colon
> Adenocarcinoma that has a loss of expression as a negative tissue control
> for all four antibodies (MLH1, MSH2, MSH6, PMS2).  Stromal cells in the
> negative tissue control should have positive nuclear staining and serve as
> a positive internal control.  NordiQC doesn't have any recommendations for
> alternative tissue to use as a negative control.
>
> Anyone doing anything differently?
>
> Thanks,
>
> Scott
>
> Scott A. Lindrud, MLS(ASCP)CM CTCM
> Histopathology Technical Specialist/Cytotechnologist
> Rice Memorial Hospital
> 301 Becker Ave SW
> Willmar, MN 56201
> WP(320)231-4406
> Fax(320)231-4861
> scott.lindrud at rice.willmar.mn.us
>
>
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