[Histonet] Special stains and hoods

Morken, Timothy Timothy.Morken at ucsf.edu
Wed Aug 22 10:47:09 CDT 2018

I guess the question is, who told you this and what is their reference?

Most special stains don't require a hood. Only those that have volatile chemicals or odors that may be offensive (ie, ammonium sulfide). We have some areas we do specials without a hood. Our main special stain areas have a "comfort" hood that is not a real chemical hood but more like a hood in an oven hood in a commercial kitchen.  They are not certified or checked in any way and we do not claim they are safety hoods.

Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

-----Original Message-----
From: Laurie Colbert via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, August 21, 2018 7:34 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Special stains and hoods

I would like to know how many people perform (manual) routine special stains under a hood.  We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - but we do them on the counter, not under a hood.  We have been told the staining needs to be done under a hood, but I have never done that in my entire career.
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