[Histonet] Calretinin for Hirschsprung's
carl.hobbs at kcl.ac.uk
Mon Aug 13 14:11:34 CDT 2018
When I was doing them biopsies ( pre-operative) to establish status, we used a std histochemical method on frozen sections of biopsy material ( visualising acetylcholinesterase activity).
It used Osmium to enhance /darken positivity so, NOT recommended these days.
I recall somebody leaving the lid off the Osmium ( stored at 4C)…..the fridge plastic lining was BLACK the next day!
So, any antibody that identifies nerve fibres and ganglion cells should be equally effective, using IHC/IF?
Sure, interpretation is critical ( identifying presence/absence of ganglion cells and "significant" increase in lamina propria of nerve fibres as compensatory mechanism)
There must be an internationally recognised/accepted protocol?
Which technique does Great Ormond Street Hospital/St Thomas' Hospital use, these days?
Carl Hobbs FIBMS
Histology and Imaging Manager
Guys Campus, London Bridge
Kings College London
020 7848 6813
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