[Histonet] Attwood tip

[Terri Braud]

Have you tried Polyphenyl Yellow 8G instead of Tartrazine?  Just a suggestion

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal


Today's Topics:
   1. Attwood's tips please (Finlay Finlay)

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Message: 1
Date: Mon, 9 Apr 2018 12:40:45 +0000
From: Finlay Finlay <Finlay.Finlay at glasgow.ac.uk>
Subject: [Histonet] Attwood's tips please
Hello,
I'm trying to work up an Attwood's  method but would be keen to get a guide on time for the differentiation step in tartrazine solution. The method I'm using suggests 20 minutes at the longest with regular microscope checks. I'm currently at about 4 hours with only slight loss of colour in the RBC's. I know that the tartrazine solution will work eventually as the slides I left in over the weekend became fully decolourised but those time scales are unworkable.
Thanks,

Finlay Finlay

Senior Histology Technician
Forensic Medicine and Science
School of Medicine
College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building

Direct Line: +44 (0) 141 3303443
E-mail: finlay.finlay at glasgow.ac.uk<mailto:finlay.finlay at glasgow.ac.uk>

The University of Glasgow Charity Number SC004401

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End of Histonet Digest, Vol 173, Issue 7
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