[Histonet] Attwood's tips please

[Finlay Finlay]

Hello,
I'm trying to work up an Attwood's  method but would be keen to get a guide on time for the differentiation step in tartrazine solution. The method I'm using suggests 20 minutes at the longest with regular microscope checks. I'm currently at about 4 hours with only slight loss of colour in the RBC's. I know that the tartrazine solution will work eventually as the slides I left in over the weekend became fully decolourised but those time scales are unworkable.
Thanks,

Finlay Finlay

Senior Histology Technician
Forensic Medicine and Science
School of Medicine
College of Medical, Veterinary and Life Sciences
University of Glasgow
Joseph Black building

Direct Line: +44 (0) 141 3303443
E-mail: finlay.finlay at glasgow.ac.uk<mailto:finlay.finlay at glasgow.ac.uk>

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