[Histonet] Running ISH on the Ventana Ultra Stainers

[Eddie Martin]

Hi Miranda,

For starters, perform a Decontamination on your Ultras, don't dilute your probes. At our lab, we manually dispense the probes but a prep kit is fine.  And also use a toothpick to clear out any gunk in your detection dispensers prior to doing a run. Also, check if your ultras are on battery backups providing sufficient run time amperage in case of power failures or abnormalities. Also moving your ultras or the instrument not being level can create issues. And check that you lo of charged slides are staining well across several antibodies. 

If all that is ok, then try out your protocol again and see if you get adequate staining quality. 

Hope this helps to get you started!

Eddie Martin, HT,HTL,QIHC
IHC Histotechnologist III
The Joint Pathology Center
Walter Reed National Military Medical Center
Bethesda, MD

Sent from my iPhone

> On Sep 19, 2017, at 1:25 PM, Miranda Giorgi <mgiorgi at incdx.com> wrote:
> 
> Hello Histonetters,
> 
> Our lab is in the process of trying to validate Kappa, Lambda and EBER ISH on the Ventana Ultra stainers.  We have run these probes on the XT in the past with good results, but unfortunately staining on the Ultras is either poor or non-existent.  We have tried several protocol modifications with very little luck.  Does anybody out there have experience validating these tests on the Ultras?  We would greatly appreciate any advice on getting these to work.
> 
> Thanks in advance!
> 
> 
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