[Histonet] FW: cutting a 5um frozen section from a 50um section of brain
Dessasau III, Evan
eddessa at emory.edu
Fri Oct 6 17:29:39 CDT 2017
Hi Loralei, I will see If I can find someone with a sliding microtome. Are you using OCT to block the 50 um section? How do you ensure the section stays flat while blocking/freezing?
Thank you for your reply !
From: Loralei Dewe [mailto:lldewe at gmail.com]
Sent: Friday, October 06, 2017 6:13 PM
To: Dessasau III, Evan <eddessa at emory.edu>
Subject: Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain
I would use a sliding microtome and freeze the 50 um sections solid with dry ice. I used to do sections of mouse brain that way.
On Oct 6, 2017 9:10 AM, "Dessasau III, Evan via Histonet" <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:
Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section from 50um sections of brain. Every time I think I have the tissue flat the sections are never in the same plane. I found a wonderful book in pdf format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters) online with lots of wonderful tips but I'm having no luck implementing the tips. Has anyone ever tried this? Any help is GREATLY appreciated.
E-van D. Dessasau, III, HTL(ASCP)cm
Supervisor, Histology Division of Pathology
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