[Histonet] Help with preparing slides of baby guppies

J B craigak12 at gmail.com
Wed May 31 11:50:55 CDT 2017


Trying to fully understand. You need someone to prepare these slides again
for you?  Great quality work, you provide the specimen?  Let me know, I
would love to learn more.



On Wed, May 31, 2017, 9:13 AM Dearolf, Jenn via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello!
> My name is Jenn Dearolf, and I am a professor in the Biology Department at
> Hendrix College, a small liberal arts college in Conway, AR.  I have
> written this list before to get advice about how to prevent freezing
> artifact in small muscle samples (Thanks!), but today, I have a completely
> different need.
> In our Zoology course at Hendrix, we use slides that have numerous serial
> sections (7 to 10 microns thickness) of a baby guppy on them that have been
> stained with H & E.  And, a box of slides, ranging from 8 to 12 slides, is
> the entire guppy sectioned from the tip of its nose to the tip of its
> tail.  However, these slides are very old, and over the years, the mounting
> media has pulled away from the sections.  In addition, numerous slides have
> been broken or lost.
> Unfortunately, no one in my Department now has the necessary skills to
> produce these slides for our students.  I was wondering if anyone on the
> list knew of a facility that could produce these slides.  I think we should
> be able to provide the baby guppies.  I would just have to get approval
> from our IACUC.
> I also have a very old paper that discusses the steps that were necessary
> to produce the slides.  I am happy to share the methodology with anyone
> that could help us, and we could discuss what we would need to do at
> Hendrix and which steps would need to be performed at your facility.
> I appreciate any advice folks are willing to share.  Thanks for your time
> and consideration.
> Sincerely,
> Jenn
> Jennifer Dearolf, Ph.D.
> Professor and Chair
> Biology Department
> Hendrix College
> 1600 Washington Ave.
> Conway, AR 72032
> (501) 450-4530 (office)
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Have a great day!

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