[Histonet] A Question About Paraffin Times
Kari Simeone
KSimeone at leavittmgt.com
Thu May 18 12:34:28 CDT 2017
I agree with Tony. I have on many occasions left the block baskets sit at room temperature (sat them in an OFF oven) and then turned the oven on to temp and re-embedded the tissue with no issues. You could also just return the basket to the warming tray until they are warm again to embed.
Kari M Simeone
Histology/Immunohistochemistry Specialist Supervisor
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Subject: Histonet Digest, Vol 162, Issue 17
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Today's Topics:
1. Histotech and Histology Supervisor job openings in
Birmingham, AL (Brannon Owens)
2. Re: A Question About Paraffin Times (Tony Henwood (SCHN))
3. Re: A Question About Paraffin Times (Garrey Faller)
4. Decal Solution (Diana Martinez-Longoria)
5. Histo job opportunity in a GI lab-Oak Lawn Illionios
(dusko trajkovic)
6. Grosser Needed in Southern California (Paula)
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Message: 1
Date: Wed, 17 May 2017 19:40:39 +0000
From: Brannon Owens <brannon at alliedsearchpartners.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Histotech and Histology Supervisor job openings in
Birmingham, AL
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We are currently seeking candidates for several openings in the Birmingham, AL area. Relocation assistance is offered!
Position 1: Histology Supervisor (3rd shift)
Position 2: Histotechnician (1st shift)
Position 3: Histotechnician (3rd shift)
Full job descriptions available on the Allied Search Partners website as well as additional opportunities in the field of histology.
We look forward to hearing from you!
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Message: 2
Date: Wed, 17 May 2017 23:26:24 +0000
From: "Tony Henwood (SCHN)" <tony.henwood at health.nsw.gov.au>
To: P Sicurello <patpxs at gmail.com>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] A Question About Paraffin Times
Message-ID:
<0237449DE79DBC45B686AB82CDCD16FF95554CC0 at SVDCMBX-MEX008.nswhealth.net>
Content-Type: text/plain; charset="us-ascii"
It is best to remove the blocks and let them set at room temperature.
Leaving them at 60 or more degrees can adversely affect antigens.
We routinely do this with our autopsies and have not noted any issues.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, 17 May 2017 1:34 AM
To: HistoNet
Subject: [Histonet] A Question About Paraffin Times
Good Morning Listers,
I am asking the collective wisdom of the Histonet this question:
Is it better to remove baskets from the processor on Saturday morning and:
A. Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B. Leave the cassettes in molten paraffin and embed Monday morning?
I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things.
What do you think?
Thank in advance.
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872 <#>
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Message: 3
Date: Thu, 18 May 2017 07:23:50 -0400
From: Garrey Faller <garreyf at gmail.com>
To: Cindy Bird <CBird at amli-denton.com>
Cc: "Logan, Shannon" <Shannon.Logan at bellin.org>,
"histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] A Question About Paraffin Times
Message-ID: <B5A0FF7C-9EA7-446D-A328-F84E12668515 at gmail.com>
Content-Type: text/plain; charset=us-ascii
I have had to deal with this in the past and could not find an answer .
My intuition was that it can't be good to leave in hot wax. So, on the rare occasion when my only Histotech called in sick I had to take the blocks out and cool them down. When ready they were put back in the tissue embedder to heat up and everything was fine. That's my experience,
Garrey
Sent from my iPhone
> On May 16, 2017, at 2:48 PM, Cindy Bird via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> I agree?
>
> Sent from my iPhone
>
>> On May 16, 2017, at 1:28 PM, Logan, Shannon via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>>
>> Hello Paula,
>> So why must the cassettes be removed on Saturday morning if you aren't embedding until Monday morning?
>> Don't you have a "delay start" feature on your processor? Neither option A or B seem like a good thing for the tissue!
>> We time our processor to finish at 5 AM Monday when the first Histotech arrives for embedding.
>> The cassettes remain in formalin until the processor starts up on Sunday evening.
>>
>> Regards,
>>
>>
>> Shannon H. Logan B.S., HTL (ASCP)
>> Pathology Department
>>
>> Bellin Health Memorial Hospital
>> 744 South Webster Avenue
>> Green Bay, WI 54305-3400
>> 920-433-3653 X3727
>>
>>
>>
>> From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
>> Sent: Tuesday, May 16, 2017 10:34 AM
>> To: HistoNet
>> Subject: [Histonet] A Question About Paraffin Times
>>
>> Good Morning Listers,
>>
>> I am asking the collective wisdom of the Histonet this question:
>>
>>
>> Is it better to remove baskets from the processor on Saturday morning and:
>>
>> A. Let the cassettes freeze, then melt them down and embed Monday morning?
>> OR
>> B. Leave the cassettes in molten paraffin and embed Monday morning?
>>
>>
>> I am of the opinion that leaving the samples (not fatty, like breast cores)
>> in molten paraffin (62 degrees C) is bad practice, and causes them to get
>> "crunchy", among other things.
>>
>> What do you think?
>>
>> Thank in advance.
>>
>> Sincerely,
>>
>> Paula Sicurello, HTL (ASCP)CM
>>
>> Histotechnology Specialist
>>
>> UC San Diego Health
>>
>> 200 Arbor Drive
>>
>> San Diego, CA 92103
>>
>> (P): 619-543-2872 <#>
>>
>>
>>
>> *Confidentiality Notice*: The information transmitted in this e-mail is
>> intended only for the person or entity to which it is addressed and may
>> contain confidential and/or privileged material. Any review,
>> retransmission, dissemination or other use of or taking of any action in
>> reliance upon this information by persons or entities other than the
>> intended recipient is prohibited. If you received this e-mail in error,
>> please contact the sender and delete the material from any computer.
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------------------------------
Message: 4
Date: Thu, 18 May 2017 12:50:04 +0000
From: Diana Martinez-Longoria <https://urldefense.proofpoint.com/v2/url?u=http-3A__Diana.Martinez-2DLongoria-40ecrmc.org&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=ArCXb_8wVna_PBL5j1Jrb8I3sMUx6Sq4sa6dGId0DJ4&e= >
To: 'Histonet' <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Decal Solution
Message-ID:
<12B71261212BE94BB9FB7735484B9FA101014B8652 at EXMBX01.ecrmc.ci.el-centro.ca.us>
Content-Type: text/plain; charset="iso-8859-1"
Good morning everyone,
I was wondering if someone can give me advise regarding the best decal solution for bone marrow cores? Our pathologist wants to use a decal solution that has EDTA based for molecular studies (ex: suspected tumor/lymphoma etc. diagnosis). Any advice would be greatly appreciated. Thank you!
Diana Martinez-Longoria
Bachelors of Science in Biology
Histotechnician (ASCP)cm
El Centro Regional Medical Center
Phone: 760-339-7267
Fax: 760-339-4570
Email:dmlongoria at ecrmc.org
ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.
??
------------------------------
Message: 5
Date: Thu, 18 May 2017 14:25:21 +0000 (UTC)
From: dusko trajkovic <dunatrsd at sbcglobal.net>
To: "Histonet at lists.utsouthwestern.edu"
<Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Histo job opportunity in a GI lab-Oak Lawn
Illionios
Message-ID: <1790045521.669207.1495117521217 at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
If anyone is interested in a histology position in a GI lab, please let me know as soon as possible?Lab is located in Oak Lawn https://urldefense.proofpoint.com/v2/url?u=http-3A__Illinois.At&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=thbNW8AAFpx_oiEuo4V1LLh3gMir1ia2MinJ-3muB60&e= the moment I do not have details, but will be getting them soon.ThanksDusko Trajkovic760-390-3478
------------------------------
Message: 6
Date: Thu, 18 May 2017 09:54:56 -0700
From: "Paula" <plucas at biopath.org>
To: <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Grosser Needed in Southern California
Message-ID: <004601d2cff7$7c1701c0$74450540$@biopath.org>
Content-Type: text/plain; charset="us-ascii"
Hello,
We need a true PA (Pathologist Assistant) or a highly qualified Grossing
person that can do hospital work. Full time or Part Time options. If
interested, please contact Jason Cochran (Administrator) for more
information.
Bio-Path Medical Group
Fountain Valley, CA 92708
714-433-1330
Thank you,
Paula
------------------------------
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