[Histonet] A Question About Paraffin Times

Tony Henwood (SCHN) tony.henwood at health.nsw.gov.au
Wed May 17 18:26:24 CDT 2017


It is best to remove the blocks and let them set at room temperature.

Leaving them at 60 or more degrees can adversely affect antigens.

We routinely do this with our autopsies and have not noted any issues.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, 17 May 2017 1:34 AM
To: HistoNet
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:


Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
OR
B.  Leave the cassettes in molten paraffin and embed Monday morning?


I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C)  is bad practice, and causes them to get "crunchy", among other things.

What do you think?

Thank in advance.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>



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