[Histonet] A Question About Paraffin Times
Tony Henwood (SCHN)
tony.henwood at health.nsw.gov.au
Wed May 17 18:26:24 CDT 2017
It is best to remove the blocks and let them set at room temperature.
Leaving them at 60 or more degrees can adversely affect antigens.
We routinely do this with our autopsies and have not noted any issues.
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, 17 May 2017 1:34 AM
Subject: [Histonet] A Question About Paraffin Times
Good Morning Listers,
I am asking the collective wisdom of the Histonet this question:
Is it better to remove baskets from the processor on Saturday morning and:
A. Let the cassettes freeze, then melt them down and embed Monday morning?
B. Leave the cassettes in molten paraffin and embed Monday morning?
I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things.
What do you think?
Thank in advance.
Paula Sicurello, HTL (ASCP)CM
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872 <#>
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