[Histonet] A Question About Paraffin Times

[Garrey Faller]

I have had to deal with this in the past and could not find an answer .
My intuition was that it can't be good to leave in hot wax. So, on the rare occasion when my only Histotech called in sick I had to take the blocks out and cool them down. When ready they were put back in the tissue embedder to heat up and everything was fine. That's my experience,
Garrey

Sent from my iPhone

> On May 16, 2017, at 2:48 PM, Cindy Bird via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> I agree?
> 
> Sent from my iPhone
> 
>> On May 16, 2017, at 1:28 PM, Logan, Shannon via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>> 
>> Hello Paula,
>> So why must the cassettes be removed on Saturday morning if you aren't embedding until Monday morning?
>> Don't you have a "delay start" feature on your processor? Neither option A or B seem like a good thing for the tissue!
>> We time our processor to finish at 5 AM Monday when the first Histotech arrives for embedding.
>> The cassettes remain in formalin until the processor starts up on Sunday evening.
>> 
>> Regards,
>> 
>> 
>> Shannon H. Logan  B.S., HTL (ASCP)
>> Pathology Department
>> 
>> Bellin Health Memorial Hospital
>> 744 South Webster Avenue
>> Green Bay, WI 54305-3400
>> 920-433-3653  X3727
>> 
>> 
>> 
>> From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
>> Sent: Tuesday, May 16, 2017 10:34 AM
>> To: HistoNet
>> Subject: [Histonet] A Question About Paraffin Times
>> 
>> Good Morning Listers,
>> 
>> I am asking the collective wisdom of the Histonet this question:
>> 
>> 
>> Is it better to remove baskets from the processor on Saturday morning and:
>> 
>> A. Let the cassettes freeze, then melt them down and embed Monday morning?
>> OR
>> B. Leave the cassettes in molten paraffin and embed Monday morning?
>> 
>> 
>> I am of the opinion that leaving the samples (not fatty, like breast cores)
>> in molten paraffin (62 degrees C) is bad practice, and causes them to get
>> "crunchy", among other things.
>> 
>> What do you think?
>> 
>> Thank in advance.
>> 
>> Sincerely,
>> 
>> Paula Sicurello, HTL (ASCP)CM
>> 
>> Histotechnology Specialist
>> 
>> UC San Diego Health
>> 
>> 200 Arbor Drive
>> 
>> San Diego, CA 92103
>> 
>> (P): 619-543-2872 <#>
>> 
>> 
>> 
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