[Histonet] A Question About Paraffin Times

Seeley, Heather Heather.Seeley at tenethealth.com
Tue May 16 13:48:40 CDT 2017

We do a delay protocol as well. The tissue samples will sit in formalin and start processing Sunday night for a normal amount of time to come off Monday morning. If this is not a possibility, it would be better to let them harden in the paraffin than to leave them in the hot paraffin, as this will lead to the tissue becoming brittle and very difficult to section. 

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From: P Sicurello [patpxs at gmail.com]
Sent: Tuesday, May 16, 2017 11:34 AM
To: HistoNet
Subject: [Histonet] A Question About Paraffin Times

Good Morning Listers,

I am asking the collective wisdom of the Histonet this question:

Is it better to remove baskets from the processor on Saturday morning and:

A.  Let the cassettes freeze, then melt them down and embed Monday morning?
B.  Leave the cassettes in molten paraffin and embed Monday morning?

I am of the opinion that leaving the samples (not fatty, like breast cores)
in molten paraffin (62 degrees C)  is bad practice, and causes them to get
"crunchy", among other things.

What do you think?

Thank in advance.


Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872 <#>

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