[Histonet] Intensity of Mayer's Hematoxylin.

Vanetsyan, Mariam Mariam.Vanetsyan at cshs.org
Tue Mar 28 18:01:32 CDT 2017

Good Afternoon Everyone,

I have been using Modified Mayer's Hematoxylin, preformulated as a counterstain for IHC: 5 min in HEM
                                                                                                                                                                   Followed by 10 sec rinse running DI Water
                                                                                                                                                                   20 sec in Ammonium Hydroxide (30%) diluted 5 drops in 250 mL DI Water

My question: How can I increase the intensity of BLUE color?

Thank you,
Mariam Vanetsyan
Cedars-Sinai Medical Center| 8723 Alden Dr. Los Angeles, CA 90048

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