[Histonet] Sections falling off
Pablo Sánchez Quinteiro
pablo.sanchez at usc.es
Thu Mar 23 04:36:23 CDT 2017
Yes I do... if they do not fall off in the hydration.
El 22/03/2017 a las 20:04, Ingles Claire escribió:
> Do you wash the slides in water before staining to remove the Bouin's (until the sections are no longer yellow)? I seem to remember that needs to be done.
> From: Pablo Sánchez Quinteiro via Histonet [histonet at lists.utsouthwestern.edu]
> Sent: Wednesday, March 22, 2017 1:29 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Sections falling off
> Thanks for your hint Philip,
> They are stored at 37º overnight. I learnt the problem with the trapped
> water. I am very careful with it but they keep spoiling.
> They seem transparent.
> Could I try 60 minutes at 60º oven just before xylen?
> El 22/03/2017 a las 18:53, Manfre, Philip escribió:
>> -----Original Message-----
>> From: Pablo Sánchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu]
>> Sent: Wednesday, March 22, 2017 1:39 PM
>> To: histonet at lists.utsouthwestern.edu
>> Subject: [Histonet] Sections falling off
>> Dear Histonetters,
>> I am in trouble with my paraffin sections -samples fixed in Bouin's liquid.
>> I think your sections are not completely dry, after placing them on slides. Silanized slides can trap water between the sections and the slide, almost appearing like a bulge. You may need to use the corner of a paper towel to "poke" the paraffin section and drain off the excess water. Make sure the sections look dry before starting the staining procedure. They should be transparent (translucent), not opaque (milky white).
>> That's my guess
>> Philip Manfre, HT (ASCP)
>> Associate Principal Scientist
>> Merck Research Laboratories
>> 770 Sumneytown Pike
>> West Point, PA 19486
>> 215-993-0383 (fax)
>> philip_manfre at merck.com
>> Just after the first steps: Two xylol baths and 100% ethanol most of
>> them are falling off. Slides are silanised.
>> I do not think it was an adhesion problem. What do you think about?
>> Embedding, cuttibg?
>> Thanks a lot in advance
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