[Histonet] Sections falling off

[Manfre, Philip]

You can use 60 degrees if they are not for immunohistochemistry (IHC).  If they are for IHC, you need to be more cautious with temperature.  Some antigens are adversely affected or destroyed by excessive heat.  Histochemistry should work fine heating the slides that much and that would probably aid adhesion.

Phil.

-----Original Message-----
From: Pablo Sánchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, March 22, 2017 2:29 PM
To: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Sections falling off

Thanks for your hint Philip,

They are stored at 37º overnight. I learnt the problem with the trapped 
water. I am very careful with it but they keep spoiling.

They seem transparent.

Could I try 60 minutes at 60º oven just before xylen?

Pablo


El 22/03/2017 a las 18:53, Manfre, Philip escribió:

>
> -----Original Message-----
> From: Pablo Sánchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Wednesday, March 22, 2017 1:39 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Sections falling off
>
> Dear Histonetters,
>
> I am in trouble with my paraffin sections -samples fixed in Bouin's liquid.
>
> Pablo,
> 	I think your sections are not completely dry, after placing them on slides.  Silanized slides can trap water between the sections and the slide, almost appearing like a bulge.  You may need to use the corner of a paper towel to "poke" the paraffin section and drain off the excess water.  Make sure the sections look dry before starting the staining procedure.  They should be transparent (translucent), not opaque (milky white).
> 	That's my guess
>
> Phil.
>
> Philip Manfre, HT (ASCP)
> Associate Principal Scientist
> Merck Research Laboratories
> WP81-406
> 770 Sumneytown Pike
> West Point, PA 19486
>
> 215-652-9750
> 215-993-0383 (fax)
> philip_manfre at merck.com
>
>
>
>
> Just after the first steps: Two xylol baths and 100% ethanol most of
> them are falling off. Slides are silanised.
>
> I do not think it was an adhesion problem. What do you think about?
> Embedding, cuttibg?
>
> Thanks a lot in advance
>
> Pablo
>
>
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