[Histonet] Use of sodium borohydride in immunofluorescence

Lucie Guernsey lucie.s.guernsey at gmail.com
Wed Mar 22 16:51:28 CDT 2017

Hi Bob,

Yes, thanks for looking out! We're very careful with it (used only in the
fume hood and we collect the waste for pickup) and right now I'm just
updating protocols. So many of them are outdated and missing steps or
helpful info.

Thank you, also, for the best wishes.


On Wed, Mar 22, 2017 at 7:12 AM, Bob Richmond via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> >
> > Lucie Guernsey at UC San Diego asks about sodium borohydride:
> >>I'm currently going over IHC SOPs that were written by lab members that
> have long ago moved on to other jobs. One inconsistency that I've come
> across is the use of 0.5 % sodium borohydride in immunofluorescence.<<
> Does your protocol include the information that sodium borohydride is a
> serious hazmat? Here's the Sigma-Aldrich MSDS:
> http://www.sigmaaldrich.com/catalog/product/sigma/s9125?lang=en&region=US
> >>There's a chance I have pregnancy brain....<< Best wishes to you and your
> child!
> Bob Richmond
> Samurai Pathologist
> Maryville TN
> >
> >
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