[Histonet] Sections falling off

[Pablo Sánchez Quinteiro]

Thanks for your reply Jeanine,

Sections are 8 microns thick (mouse brain) and they are stored overnight 
at 37ºC

Pablo



El 22/03/2017 a las 18:51, Sanders, Jeanine (CDC/OID/NCEZID) escribió:
> Hi Pablo.
>
> How thick are your sections and how long and at what temperature do you heat the slides before beginning de-paraffinizing?
>
> Jeanine H. Sanders
> Infectious Diseases Pathology Branch
> Centers for Disease Control and Prevention
> 1600 Clifton Rd., NE MS-G32
> Atlanta, GA 30329
>
> -----Original Message-----
> From: Pablo Sánchez Quinteiro via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Wednesday, March 22, 2017 1:39 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Sections falling off
>
> Dear Histonetters,
>
> I am in trouble with my paraffin sections -samples fixed in Bouin's liquid.
>
> Just after the first steps: Two xylol baths and 100% ethanol most of them are falling off. Slides are silanised.
>
> I do not think it was an adhesion problem. What do you think about?
> Embedding, cuttibg?
>
> Thanks a lot in advance
>
> Pablo
>
>
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