[Histonet] Histonet Digest, Vol 160, Issue 15

Wed Mar 15 12:46:39 CDT 2017

When you fix the slide in 10% formalin you will have to perform AR.

TH

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Subject: Histonet Digest, Vol 160, Issue 15

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Today's Topics:

   1. Immunoperoxidase Protocol on Cytospin (Margaryan, Naira)
   2. Re: Immunoperoxidase Protocol on Cytospin (Cartun, Richard)
   3. Congo red and LED illumination problem? (Morken, Timothy)
   4. Day Shift Histotech Job in Southern CA (Melissa Owens)
   5. Bone Processing (MICHELLE BETH TITUNICK)
   6. GSH Annual Histopalooza April 21st - April 23rd Legacy Lodge,
      Lake Lanier Islands, Georgia (Zimmerman, Billie)

----------------------------------------------------------------------

Message: 1
Date: Tue, 14 Mar 2017 18:50:45 +0000
From: "Margaryan, Naira" <NMargaryan at luriechildrens.org>
To: "histonet-bounces at lists.utsouthwestern.edu"
        <histonet-bounces at lists.utsouthwestern.edu>
Cc: histonet-request <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Immunoperoxidase Protocol on Cytospin
Message-ID:
        <34B2EDA118548A4EB35D6E650345BA6469C067C8 at SV-EX08.childrensmemorial.org>

Content-Type: text/plain; charset="utf-8"

Dear histonetters:

I never done ICC.

A scientist brought me a slide with Cytospined cells on for IHC.

I am going to fix with 10%NBF (it is only what I have now) then wash with TBST.

May I skip AR step?
May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer????

Thanks in advance,
Naira

Ranked nationally in all 10 pediatric specialties by U.S. News & World Report  (LCHOC Ver 1.0)

This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version.  (LCHOC VER 1.0)

------------------------------

Message: 2
Date: Tue, 14 Mar 2017 19:38:08 +0000
From: "Cartun, Richard" <Richard.Cartun at hhchealth.org>
To: "Margaryan, Naira" <NMargaryan at luriechildrens.org>
Cc: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Immunoperoxidase Protocol on Cytospin
Message-ID:
        <9215BD4B0BA1B44D962A71C758B68D2E95401DE3 at HHCEXCHMB03.hhcsystem.org>
Content-Type: text/plain; charset="us-ascii"

Since you only have 1 slide I would recommend following your regular protocol that you use for formalin-fixed, paraffin-embedded tissue.  I would use heat retrieval if that's what you use for FFPE tissue.  However, if you use enzyme digestion on FFPE tissue I would "not" digest your cytospin slide.  If you don't get any immunoreactivity it could be a "False Negative" due to pre-analytical variables.  I think it can be interesting to try experiments like this, but it's not the way to do IHC testing.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Margaryan, Naira [mailto:NMargaryan at luriechildrens.org]
Sent: Tuesday, March 14, 2017 3:24 PM
To: Cartun, Richard
Subject: RE: Immunoperoxidase Protocol on Cytospin

cytoplasmic staining for Nodal protein.

thanks
________________________________________
From: Cartun, Richard [Richard.Cartun at hhchealth.org]
Sent: Tuesday, March 14, 2017 2:13 PM
To: Margaryan, Naira; histonet-bounces at lists.utsouthwestern.edu
Subject: RE: Immunoperoxidase Protocol on Cytospin

What is the target?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Margaryan, Naira via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, March 14, 2017 2:51 PM
To: histonet-bounces at lists.utsouthwestern.edu
Cc: histonet-request
Subject: [Histonet] Immunoperoxidase Protocol on Cytospin
Importance: High

Dear histonetters:

I never done ICC.

A scientist brought me a slide with Cytospined cells on for IHC.

I am going to fix with 10%NBF (it is only what I have now) then wash with TBST.

May I skip AR step?
May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer????

Thanks in advance,
Naira

Ranked nationally in all 10 pediatric specialties by U.S. News & World Report  (LCHOC Ver 1.0)

This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version.  (LCHOC VER 1.0)

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------------------------------

Message: 3
Date: Tue, 14 Mar 2017 19:42:36 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Congo red and LED illumination problem?
Message-ID:
        <761E2B5697F795489C8710BCC72141FF8E5C19DF at ex07.net.ucsf.edu>
Content-Type: text/plain; charset="us-ascii"

Something new to me... A pathologist said he is getting reports that LED illumination on newer microscopes is leading to faint or even false negatives for congo red amyloid birefringence due to the difference in spectrum between LED and tungsten filament lamps. Has anyone else noticed this?

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

------------------------------

Message: 4
Date: Wed, 15 Mar 2017 00:47:09 +0000
From: Melissa Owens <melissa at alliedsearchpartners.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Day Shift Histotech Job in Southern CA
Message-ID: <D4EE0747.35EE7%melissa at alliedsearchpartners.com>
Content-Type: text/plain; charset="us-ascii"

Hello Histoland,

I have a Day Shift Histotech opportunity in Southern California for full time permanent employment. Please contact me directly for more details.

Melissa Owens
President, Laboratory Staffing
Allied Search Partners

T: 888.388.7571 ext. 102

F: 888.388.7572

------------------------------

Message: 5
Date: Wed, 15 Mar 2017 09:58:08 -0400
From: "MICHELLE BETH TITUNICK" <mbt137 at psu.edu>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Bone Processing
Message-ID: <1489586288l.21430310l.0l at psu.edu>
Content-Type: text/plain; charset=utf-8

Hello.  Does anyone have a processing protocol for formalin fixed decalcified
rat bones using an automated processor?  I've been doing it manually with a
vacuum oven and it takes 14-16 hours to complete.  I don't have a vacuumed
section on my automatic processor.

Also any tips besides what I've read in the archives for immunohistochemistry?

Thanks so much.

Michelle B. Titunick

PhD Candidate, Anatomy
The Pennsylvania State University College of Medicine
mbt137 at psu.edu
(973) 715-9831

------------------------------

Message: 6
Date: Wed, 15 Mar 2017 15:21:13 +0000
From: "Zimmerman, Billie" <BZIMMERM at augusta.edu>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] GSH Annual Histopalooza April 21st - April 23rd
        Legacy Lodge, Lake Lanier Islands, Georgia
Message-ID:
        <MWHPR03MB2607CB3447F7389662F02637CB270 at MWHPR03MB2607.namprd03.prod.outlook.com>

Content-Type: text/plain; charset="us-ascii"

The Georgia Society for Histology Histopalooza is just around the corner!! Calling all procrastinators to register for the event.
 It's cheap, cheap, cheap compared to other CEU events.  I spoke with Claudia Faulkenberry this morning.
 She will be speaking about the  hot, hot antibody PDL1. (no, it ain't cheap )
It's on television commercials so the general public is asking about it.  She will tell us the uses for this antibody as well as the various clones available.   Companion diagnostics is the new  buzz word for PDL1. Thank you Claudia.

See you at Lake Lanier,
Billie Zimmerman MT(ASCP)QIHC

------------------------------

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End of Histonet Digest, Vol 160, Issue 15
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