[Histonet] Immunoperoxidase Protocol on Cytospin

[Margaryan, Naira]

Dear histonetters:

I never done ICC.

A scientist brought me a slide with Cytospined cells on for IHC.

I am going to fix with 10%NBF (it is only what I have now) then wash with TBST.

May I skip AR step?
May I use my usual IHC protocol and reagents which I usually use for FFPE tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer????

Thanks in advance,
Naira


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