[Histonet] TUNEL

[Caroline Miller]

I have done that a lot and I don't see why it would not pick up hx or DAPI.
Was there anything left on the slide? Maybe it was mounted in aqueous
already??

mills

On Fri, Mar 3, 2017 at 11:42 AM, Bernice Frederick via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello all.
> Would there be any reason that slides stained with TUNEL (ISH) would not
> pick up the Mayers counterstain? We use it for IHC and it is fine. Mayers
> for 7 minutes and then ammonia water for 1 minute. We do that rather than
> rinse for 15 minutes and there are usually no issues. Could a reagent in
> the stain be causing it? DAPI is used...
> Bernice
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-frederick at northwestern.edu<mailto:b-frederick at northwestern.edu>
>
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297