[Histonet] TUNEL

Bernice Frederick b-frederick at northwestern.edu
Fri Mar 3 13:42:47 CST 2017

Hello all.
Would there be any reason that slides stained with TUNEL (ISH) would not pick up the Mayers counterstain? We use it for IHC and it is fine. Mayers for 7 minutes and then ammonia water for 1 minute. We do that rather than rinse for 15 minutes and there are usually no issues. Could a reagent in the stain be causing it? DAPI is used...

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
b-frederick at northwestern.edu<mailto:b-frederick at northwestern.edu>

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