[Histonet] IHC sections adherence

Thu Jul 13 12:33:38 CDT 2017

Hi Greg, I use BONDS and Apex slides as well. What we do with tissue that washes (usually heavy tumor and some harsher stains like CD34) is leave the tissue unbaked at room temp for 12 hours and then bake at 60 degrees for 30 minutes. I don't know if you are including your control tissue on the same slide (we do) but we also cut a separate slide with just patient sections and that seems to help with extra paraffin runoff from the control tissue underneath the patient tissue. We've been doing this successfully for years.
I hope this is helpful, I totally understand your frustration! 

Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

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Subject: Histonet Digest, Vol 164, Issue 9

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Today's Topics:

   1. Re: Section adherence issues (IHC) (Walter Benton)
   2. Permanent/Full Time Histotech Job in Panama City, FL
      (Melissa Owens)
   3. New Lab Related Blog Post (Lester Raff MD)

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Message: 1
Date: Wed, 12 Jul 2017 17:37:38 +0000
From: Walter Benton <wbenton at cua.md>
To: "Hujet, Matthew" <https://urldefense.proofpoint.com/v2/url?u=http-3A__Matthew.Hujet-40ssmhealth.com&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=F1TSqbvH_gNcZCNxbzfj4YvTHIhCktr_3GoGHgornJM&e= >, Greg Dobbin
        <https://urldefense.proofpoint.com/v2/url?u=http-3A__greg.dobbin-40gmail.com&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=VTefCjSt2cBRdRq7WmFsFV-QelqPZmBxp9PaxdoXh20&e= >
Cc: "kgeveleigh at ihis.org" <kgeveleigh at ihis.org>,
        "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Section adherence issues (IHC)
Message-ID: <816e042155824c4193f79c50c1461b48 at MAIL01.GCU-MD.local>
Content-Type: text/plain; charset="us-ascii"

Thanks. This is helpful information.

-----Original Message-----
From: Hujet, Matthew [mailto:Matthew.Hujet at ssmhealth.com]
Sent: Wednesday, July 12, 2017 1:35 PM
To: Walter Benton <wbenton at cua.md>; Greg Dobbin <https://urldefense.proofpoint.com/v2/url?u=http-3A__greg.dobbin-40gmail.com&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=VTefCjSt2cBRdRq7WmFsFV-QelqPZmBxp9PaxdoXh20&e= >
Cc: kgeveleigh at ihis.org; histonet at lists.utsouthwestern.edu
Subject: RE: [Histonet] Section adherence issues (IHC)

We have had really good luck with both the TOMO and Millennia 2000 adhesion slides. We initially tried using the TOMO slides for everything (IHC, special stains, and H&E's), but found that there was too much background staining on the specials and H&E's. For IHC, the TOMO slides were great. We then switched to the Millennia 2000 for everything, and they have been working really well across the board. With regards to just IHC, I personally feel that the TOMO slides are better, but if you only want one type of glass in your lab, the Millennia 2000 are the way to go.

Matthew Hujet
Histology Technical Specialist
SSM Health St. Mary's Hospital - Madison
700 S. Park St.
Madison, WI 53715

Please note my new email address: Matthew.Hujet at ssmhealth.com

-----Original Message-----
From: Walter Benton [mailto:wbenton at cua.md]
Sent: Wednesday, July 12, 2017 10:34 AM
To: Greg Dobbin <https://urldefense.proofpoint.com/v2/url?u=http-3A__greg.dobbin-40gmail.com&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=VTefCjSt2cBRdRq7WmFsFV-QelqPZmBxp9PaxdoXh20&e= >
Cc: kgeveleigh at ihis.org; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Section adherence issues (IHC)

Greg,

We are running into a similar issue on the Biocare Nemesis platform and the support there suggest humidity to be the issue. Our sections stay adhered to the slides through depar, antigen retrieval, but appear to fall off during the staining process. It is not consistent. We are going to try some other slides and see if the issue persists. I'd be interested to hear your findings and resolution.

Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061

-----Original Message-----
From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, July 12, 2017 11:09 AM
To: histonet at lists.utsouthwestern.edu
Cc: kgeveleigh at ihis.org
Subject: [Histonet] Section adherence issues (IHC)

Hi Folks,
I haven't been on here much lately but it is nice to know that we are all here for each other when needed!

My problem is section adherence during IHC staining. And it is not all of the time it is intermittent; so not all of the time and not on all slides when it does occur.

Background:
We have a Bond-III immunostainer and we use the Leica Apex charged slides for our IHC stains. We have no additives in our water baths. Our sections drain in a stand and then any trapped water under the section is either flicked or wicked away as needed prior to placing the slides in a rack in a
60 C oven for 30 mins prior to staining.  We do not do on board baking (primarily because on board baking is only 10 mins long and with the section lifting issue we want longer).

Some of the specimen types are more susceptible it would seem. Cervix LEEP specimens tend to be bad, we use a 4mm punch to obtain some of our control tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad one and sometimes our ER/PR control sections (but not always). Fine needle cores where a lot of tumour is present tend to be bad (again not always).

We have tried baking longer, we turn ourselves inside out trying to get all of the water out from under the sections, we have tried charged slides from another manufacturer. I have looked at the HIER protocols and none are extraordinary in nature. We use very clean covertiles (no scratches or blemishes). Our specimens are fixed for 24hrs before processing. We use the same slides for Special Stains and don't have this issue there.

I need some new suggestions to try! All ideas welcome.
Cheers,
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE      C0A 1P0

*Everything in moderation...even moderation itself**!* _______________________________________________
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------------------------------

Message: 2
Date: Wed, 12 Jul 2017 19:59:14 +0000
From: Melissa Owens <melissa at alliedsearchpartners.com>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Permanent/Full Time Histotech Job in Panama City,
        FL
Message-ID: <D58BF7C8.413F4%melissa at alliedsearchpartners.com>
Content-Type: text/plain; charset="us-ascii"

Hello All,

I have a full time/permanent position for a Histotech in Panama City, FL. This is a Monday-Friday Day Shift opportunity and I am looking for a long term permanent candidate. Please let me know if you are interested and I will provide more information. Thank you!

Melissa Owens
President, Laboratory Staffing
Allied Search Partners

Direct Line: 407.413.9117

Toll Free: 888.388.7571 ext. 102

F: 888.388.7572

------------------------------

Message: 3
Date: Thu, 13 Jul 2017 11:43:46 +0000
From: Lester Raff MD <LRaff at uropartners.com>
To: "Histonet (histonet at lists.utsouthwestern.edu)"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] New Lab Related Blog Post
Message-ID:
        <6347C6D2B080534F9B5C2B08436DCFAF114F70C9 at COLOEXCH01.uropartners.local>

Content-Type: text/plain; charset="us-ascii"

Good morning to all. The rains in Chicago have finally stopped.  Link for a lab blog post is below.

https://urldefense.proofpoint.com/v2/url?u=http-3A__www.chicagonow.com_downsize-2Dmaybe_2017_07_oops-2Dwe-2Ddid-2Dit-2Dagain-2Danother-2Dresearch-2Dpaper-2Dtells-2Dus-2Dpathologists-2Dsometimes-2Dhave-2Dit-2Dtough-2Dthis-2Dtime-2Dwith-2Dmelanoma_&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=BMnYbAIiXA3i7op2GB24dtCro4-e1ZM2MHR_w-FW3gc&e=

Thanks,

Lester J. Raff, MD MBA
Laboratory Medical Director

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