[Histonet] Leica SelecTech Stains

Cooper, Brian bcooper at chla.usc.edu
Mon Jul 10 10:40:10 CDT 2017

Hi Amanda, 

We used to use their Define Solution, but now we simply stain with their Hematoxylin 560 MX and Alcoholic Eosin-Y.  1% acid alcohol (5 quick dips) for differentiation and dilute ammonia water for bluing.    


Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357     Pager: 213-209-0184
bcooper at chla.usc.edu 

-----Original Message-----
From: Amanda Reichard via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Monday, July 10, 2017 6:53 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Leica SelecTech Stains (EXTERNAL EMAIL)

For those of you using the SelecTech stains...

Do you use the entire system (define and blue buffer), or just the hematoxylin and eosin?

It seems from previous posts everyone who has used it is very happy with it, I'm just curious if we can stick with a less pricey differentiation and bluing solution.

Thanks in advance

Amanda Reichard, HTL (ASCP)cm
Histology/Cytology Supervisor
Licking Memorial Health Systems
1320 W. Main St.
Newark, OH 43055
(220) 564-4163
areichard at lmhealth.org<mailto:areichard at lmhealth.org>


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