[Histonet] Leica SelecTech Stains

[Cooper, Brian]

Hi Amanda, 

We used to use their Define Solution, but now we simply stain with their Hematoxylin 560 MX and Alcoholic Eosin-Y.  1% acid alcohol (5 quick dips) for differentiation and dilute ammonia water for bluing.    

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357     Pager: 213-209-0184
bcooper at chla.usc.edu 

-----Original Message-----
From: Amanda Reichard via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Monday, July 10, 2017 6:53 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Leica SelecTech Stains (EXTERNAL EMAIL)

For those of you using the SelecTech stains...

Do you use the entire system (define and blue buffer), or just the hematoxylin and eosin?

It seems from previous posts everyone who has used it is very happy with it, I'm just curious if we can stick with a less pricey differentiation and bluing solution.

Thanks in advance

Amanda Reichard, HTL (ASCP)cm
Histology/Cytology Supervisor
Licking Memorial Health Systems
1320 W. Main St.
Newark, OH 43055
(220) 564-4163
areichard at lmhealth.org<mailto:areichard at lmhealth.org>


________________________________

This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 220-564-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, 
is for the sole use of the intended recipient(s) and may contain confidential
or legally privileged information. Any unauthorized review, use, disclosure
or distribution is prohibited. If you are not the intended recipient, please
contact the sender by reply e-mail and destroy all copies of this original message.