[Histonet] staining samples years in formalin

[Gudrun Lang]

Hi Mary Lou,
I think the problem is, that proteoglycanes will be solved by acids through
decal. Try EDTA decal or prolong the staining time in Alcianblue - maybe for
2 hours?
Try to decal your controls in the same manner and compare the results.
I don't think, that formalin fixation is the culprit, but long storage in
aequous solutions like formalin may influence watersoluble proteoglycans.

Gudrun

-----Ursprüngliche Nachricht-----
Von: Mary Lou Norman via Histonet [mailto:histonet at lists.utsouthwestern.edu]

Gesendet: Mittwoch, 18. Januar 2017 15:01
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] staining samples years in formalin

Dear Histonetters,
My samples have been in formalin up to 3 years and there have been
complaints about poor staining. Please help me with solutions if there are
any. My controls have not been in formalin as long. The complaint has been
with the Alcian Blue pH2.5 and Saf O specifically.
The samples are stifles so I need to decal them also. I use formic/na
citrate.
Any and all comments, suggestions are much appreciated. Thank you.
Mary Lou Norman

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet