[Histonet] FYI: DAB vs 88% formic acid

Tyrone Genade tgenade at gmail.com
Tue Feb 21 12:52:34 CST 2017


In case you are curious DAB on IHC sections is resistant to 88% formic acid.

If you want to know why I would put a DAB stained section in formic acid, I
have conformation specific antibody that I want to use in a
double-labelling experiment using an antibody needing formic acid epitope

Incidentally, having trouble matching antibodies for double labelling? This
might be useful: Antibody Elution Method for Multiple Immunohistochemistry
on Primary Antibodies Raised in the Same Species and of the Same Subtype
http://journals.sagepub.com/doi/10.1369/jhc.2009.953240 I haven't tried
this method yet but I will...

Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
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