[Histonet] Histonet Digest, Vol 159, Issue 10

Sun Feb 12 12:19:40 CST 2017

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    On Saturday, February 11, 2017 1:00 PM, "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu> wrote:

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Today's Topics:

  1. need automation system for fluorescent Multiplex IHC (Maria Mejia)
  2. New lab (Emily Horst)
  3. Selling a Ventana Discovery XT (Allan Wang)
  4. Re: need automation system for fluorescent Multiplex IHC
      (Maria Mejia)
  5. BD PROBETEC (Baldwin, Kathy)
  6. PRN Histo position (Steven Crochiere)
  7. Fat or Breast & Lipoma Processing Schedule Sought (ian bernard)
  8. Re: Fat or Breast & Lipoma Processing Schedule Sought
      (Rene J Buesa)
Dear All,

I work in a research lab where the focus is on early stages of Alzheimer’s Disease.
We are seriously considering using an automated staining system for fluorescent Multiplex
IHC, for the simultaneous detection of multiple (2-6 or more) proteins of interest in 
FFPE brain sections.

However, our lab has never used an automated staining system before, so we lack
the abilities and experience to make the correct choice for our lab’s needs.

Cost; the price of a USED system, since we are a research lab since a new system is more
expensive, user friendly & size of system are of course major considerations as well 
as the consumables that go along with the system e.g. reagents, containers, & anything else
to consider as well.

Please, I’m reaching out to anyone (system users & vendors) who can help our lab make a smart choice.
I would be most grateful for any recommendations or suggestions. I very much look forward to hearing
from you.

Best
Maria Mejia
Lead Histologist
UCSF 
Memory & Aging Center

Hi everyone,

I am in the process of setting up a new lab. We will process FNA specimens
as cell blocks and then treat them as regular histology specimens.

I am curious as to finding a proper VIP schedule for processing these
specimens. Any suggestions??

Thanks in advance,

Emily Horst

-- 
Emily Horst, HT(ASCP)
Office Manager
Interventional Pathology of Ohio, LLC
Kettering OH 45429
937-297-0028

Hello list,

The last message reminded me:

My lab is upgrading from the Discovery XT to the Ultra so we're selling the
XT. We're offering it for a great price compared to new. It'll come with
the computer, slide label printer, accessories, and all the reagents -
everything needed to get started. It's only been used for 7 months and is
still under the original first year service contract (doubt it will
transfer though)

If anyone is interested, I'd be happy to discuss the pros and cons of the
system. It is located in Rockville, MD.

Here is an example of a double stain:
http://www.biomax.us/images/ER-Brown_HER2-Purple_core.jpg

Allan Wang
Lab Manager
US Biomax

I forgot to mention in my email below our sections will be paraffin for Multiplex
fluorescent.

Thank you
> On Feb 10, 2017, at 10:43 AM, Maria Mejia <mbmphoto at gmail.com> wrote:
> 
> Dear All,
> 
> I work in a research lab where the focus is on early stages of Alzheimer’s Disease.
> We are seriously considering using an automated staining system for fluorescent Multiplex
> IHC, for the simultaneous detection of multiple (2-6 or more) proteins of interest in 
> FFPE brain sections.
> 
> However, our lab has never used an automated staining system before, so we lack
> the abilities and experience to make the correct choice for our lab’s needs.
> 
> Cost; the price of a USED system, since we are a research lab since a new system is more
> expensive, user friendly & size of system are of course major considerations as well 
> as the consumables that go along with the system e.g. reagents, containers, & anything else
> to consider as well.
> 
> Please, I’m reaching out to anyone (system users & vendors) who can help our lab make a smart choice.
> I would be most grateful for any recommendations or suggestions. I very much look forward to hearing
> from you.
> 
> Best
> Maria Mejia
> Lead Histologist
> UCSF 
> Memory & Aging Center
> 
> 

Hi Histonetters!!
              We have a probetec unit that our histology department is willing to sell.  Below is conformation of the manufacturer date.  It was taken out of service this past fall and was in full working order.  It does have a water bath and heat block (the heat block is having issues maintaining a temp).  The model is BD Probetec ET, serial number: PT 2782.  I will get ahold of our materials management department if this is something any of you are interested in.

Thanks
S. Kathy Baldwin
Histology/Cytology Supervisor
Memorial Hospital and Health Care Center
800 West 9th St.
Jasper, Indiana  47546
Office 812-996-0210
Fax 812-996-0232

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Currently seeking an experienced HT for dermpath/MOHS laboratory in
Springfield, MA to cover for absences and possibility to become FT  in
future.

Contact:

Terry Molnar

New England Dermatology

3455 Main St

Springfield, MA 01107

tmolnar at nedlc.com <mailto:tmolnar at nedlc.com> 

(413)733-9600 x319

We use Safe Clear 11 (a Xylene sub) as the clearing agent on our routine
processing schedule; with no intent to resort back to Xylene.  

Since we are taking in an influx of lipoma and breast cases, we are seeking
a separate tissue processing schedule.  Thus looking for an overnight
schedule template that we can validate.

Are there any histonetters with an overnight schedule that they are willing
to share as our template to begin validation.  Also, any reference source
you can point too along these lines will be greatly appreciated.

V/r

IB

You can find all my xylene-free processing schedules at the HistoNet archives. They use 2-propanol → mineral oil → paraffin.René  

    On Saturday, February 11, 2017 9:44 AM, ian bernard via Histonet <histonet at lists.utsouthwestern.edu> wrote:

 We use Safe Clear 11 (a Xylene sub) as the clearing agent on our routine
processing schedule; with no intent to resort back to Xylene.  

Since we are taking in an influx of lipoma and breast cases, we are seeking
a separate tissue processing schedule.  Thus looking for an overnight
schedule template that we can validate.

Are there any histonetters with an overnight schedule that they are willing
to share as our template to begin validation.  Also, any reference source
you can point too along these lines will be greatly appreciated.

V/r

IB

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