[Histonet] hand staining immunos

Ranna Mehta ranna0726 at gmail.com
Thu Feb 9 13:32:49 CST 2017 

Hi Jennifer,

  How many slides are you planning to stain in routine?  Once you will
understand the basic, it will be a very interesting field. If you like, I
can help you to walk through process with step by step. send me a message
through my email.All the best and thanks.

Regards
Ranna

On Thu, Feb 9, 2017 at 12:00 PM, <histonet-request at lists.utsouthwestern.edu>
wrote:

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> Today's Topics:
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>    1. Looking for a Histology Workshop (Jessica Riggleman)
>    2. Re: Looking for a Histology Workshop (Jessica Riggleman)
>    3. hand staining immunos (Jennifer)
>    4. Re: hand staining immunos (Rene J Buesa)
>    5. Re: hand staining immunos (Walter Benton)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 8 Feb 2017 18:07:24 +0000
> From: Jessica Riggleman <jriggleman at globusmedical.com>
> To: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Looking for a Histology Workshop
> Message-ID:
>         <D0AEE53F1F084340888BEB10C81DE9B295C77FAC at SW10EXM01.gminc.local>
> Content-Type: text/plain; charset=UTF-8
>
>
> Hi Everyone,
>
> I am looking for workshop or class for Histology/Histotechnology that
> lasts anywhere from 1 week to 3 months in the U.S.
> Preferably in-person and not online.
>
> If you have any suggestions or contacts, please let me know.
>
> Thank You,
> Jessica
>
> _____________________________________________________________________
>
> Jessica Riggleman | Research Associate
>
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> website: http://www.globusmedical.com | email:
> jriggleman at globusmedical.com<mailto:jriggleman at globusmedical.com>
> An ISO 13485 Registered Company
>
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> Inc. for any loss or damage arising in any way from the receipt or use
> thereof.
>
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>
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> ------------------------------
>
> Message: 2
> Date: Wed, 8 Feb 2017 21:33:47 +0000
> From: Jessica Riggleman <jriggleman at globusmedical.com>
> To: Esther C Peters <epeters2 at gmu.edu>
> Cc: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Looking for a Histology Workshop
> Message-ID:
>         <D0AEE53F1F084340888BEB10C81DE9B295C780C0 at SW10EXM01.gminc.local>
> Content-Type: text/plain; charset=UTF-8
>
> Hi Esther,
>
> We are looking for basic training to include study design, sectioning raw
> specimens for processing (i.e., how large should my specimen be?),
> processing techniques (fixation, etc.), special stains, and slide reading.
> This is not for HT/HTL exam preparation.
>
> We would prefer non-human tissues, as we typically work with large sheep
> and rabbit specimens. Only two students.
>
> Thank You,
> Jessica
>
>
> From: Esther C Peters [mailto:epeters2 at gmu.edu]
> Sent: Wednesday, February 08, 2017 4:18 PM
> To: Jessica Riggleman <jriggleman at globusmedical.com>
> Cc: histonet at lists.utsouthwestern.edu
> Subject: Re: Looking for a Histology Workshop
>
> Is this for human or non-human tissues? How many students?
>
> Esther
>
>
> _____________________________________________________________________
>
> Jessica Riggleman  | Research Associate
>
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
>
> Confidentiality Note:  This email is confidential and intended solely for
> the use of the individual to whom it is addressed. If you are not the
> intended recipient, be advised that you have received this email in error
> and that any use, dissemination, forwarding, printing, or copying of this
> email is strictly prohibited. If you have received this email in error
> please contact the sender. Any views or opinions presented are solely those
> of the author and do not necessarily represent those of Globus Medical,
> Inc. Although this email and any attachments are believed to be free of any
> virus or other defects which might affect any computer or IT system into
> which they are received, no responsibility is accepted by Globus Medical,
> Inc. for any loss or damage arising in any way from the receipt or use
> thereof.
>
>
> ________________________________________
> From: Jessica Riggleman <jriggleman at globusmedical.com>
> Sent: Wednesday, February 8, 2017 4:12:20 PM
> To: Esther C Peters
> Cc: histonet at lists.utsouthwestern.edu
> Subject: RE: Looking for a Histology Workshop
>
> Hi Esther,
>
> We are looking for basic training to include study design, sectioning raw
> specimens for processing (i.e., how large should my specimen be?),
> processing techniques (fixation, etc.), special stains, and slide reading.
> This is not for HT/HTL exam preparation.
>
> Thank You,
> Jessica
>
> ________________________________________
>
> Jessica Riggleman  | Research Associate
>
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> An ISO 13485 Registered Company
>
> website | map | email
>
>
>
>
>
> Confidentiality Note:  This email is confidential and intended solely for
> the use of the individual to whom it is addressed. If you are not the
> intended recipient, be advised that you have received this email in error
> and that any use, dissemination, forwarding, printing, or copying of this
> email is strictly prohibited. If you have received this email in error
> please contact the sender. Any views or opinions presented are solely those
> of the author and do not necessarily represent those of Globus Medical,
> Inc. Although this email and any attachments are believed to be free of any
> virus or other defects which might affect any computer or IT system into
> which they are received, no responsibility is accepted by Globus Medical,
> Inc. for any loss or damage arising in any way from the receipt or use
> thereof.
> From: Esther C Peters [mailto:epeters2 at gmu.edu]
> Sent: Wednesday, February 08, 2017 3:53 PM
> To: Jessica Riggleman <jriggleman at globusmedical.com>
> Subject: Re: Looking for a Histology Workshop
>
> Hi Jessica,
>
> It might help everyone to know exactly what you want to cover in the
> workshop or class. Do you need basic training, preparation for the HT/HTL
> exam, slide preparation, special stains, or slide reading? What is your
> goal?
>
> Esther
>
> Esther C. Peters, Ph.D.
> George Mason University
> Fairfax, VA 22030-4444
> ________________________________________
> From: Jessica Riggleman via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Wednesday, February 8, 2017 1:07:24 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Looking for a Histology Workshop
>
>
> Hi Everyone,
>
> I am looking for workshop or class for Histology/Histotechnology that
> lasts anywhere from 1 week to 3 months in the U.S.
> Preferably in-person and not online.
>
> If you have any suggestions or contacts, please let me know.
>
> Thank You,
> Jessica
>
> _____________________________________________________________________
>
> Jessica Riggleman | Research Associate
>
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> website: http://www.globusmedical.com | email:
> jriggleman at globusmedical.com<mailto:jriggleman at globusmedical.com>
> An ISO 13485 Registered Company
>
> Confidentiality Note:  This email is confidential and intended solely for
> the use of the individual to whom it is addressed. If you are not the
> intended recipient, be advised that you have received this email in error
> and that any use, dissemination, forwarding, printing, or copying of this
> email is strictly prohibited. If you have received this email in error
> please contact the sender. Any views or opinions presented are solely those
> of the author and do not necessarily represent those of Globus Medical,
> Inc. Although this email and any attachments are believed to be free of any
> virus or other defects which might affect any computer or IT system into
> which they are received, no responsibility is accepted by Globus Medical,
> Inc. for any loss or damage arising in any way from the receipt or use
> thereof.
>
> Disclaimer
>
> The information contained in this communication from the sender is
> confidential. It is intended solely for use by the recipient and others
> authorized to receive it. If you are not the recipient, you are hereby
> notified that any disclosure, copying, distribution or taking action in
> relation of the contents of this information is strictly prohibited and may
> be unlawful.
>
> This email has been scanned for viruses and malware, and may have been
> automatically archived by Mimecast Ltd, an innovator in Software as a
> Service (SaaS) for business. Providing a safer and more useful place for
> your human generated data. Specializing in; Security, archiving and
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> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Disclaimer
>
> The information contained in this communication from the sender is
> confidential. It is intended solely for use by the recipient and others
> authorized to receive it. If you are not the recipient, you are hereby
> notified that any disclosure, copying, distribution or taking action in
> relation of the contents of this information is strictly prohibited and may
> be unlawful.
>
> This email has been scanned for viruses and malware, and may have been
> automatically archived by Mimecast Ltd, an innovator in Software as a
> Service (SaaS) for business. Providing a safer and more useful place for
> your human generated data. Specializing in; Security, archiving and
> compliance. To find out more visit the Mimecast website.
>
> ------------------------------
>
> Message: 3
> Date: Wed, 8 Feb 2017 16:16:10 -0800
> From: "Jennifer" <histotech at dermspec.com>
> To: <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] hand staining immunos
> Message-ID: <000001d28269$b81e82f0$285b88d0$@com>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hello,
>
>
>
> My dermatopathologist wants me to start hand staining immunos  for MITF. I
> would really like some feedback about everyone's experiences/thoughts with
> manually hand staining immunos and especially with MITF. Pros and cons? I
> am
> concerned about the amount of time this will take for a tech to babysit the
> stain and also about the consistency of the quality in the end result.
> Your
> opinions are much appreciated.
>
>
>
> Thank you!
>
>
>
> Jennifer
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 9 Feb 2017 15:51:22 +0000 (UTC)
> From: Rene J Buesa <rjbuesa at yahoo.com>
> To: Jennifer <histotech at dermspec.com>,
>         "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] hand staining immunos
> Message-ID: <267846266.1416224.1486655482834 at mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
>
> You do not have to "babysit" the procedure for it has well defined/timed
> steps. You just need a timer and check the slides when required. As to
> procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren?
>
>     On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
>
>  Hello,
>
>
>
> My dermatopathologist wants me to start hand staining immunos? for MITF. I
> would really like some feedback about everyone's experiences/thoughts with
> manually hand staining immunos and especially with MITF. Pros and cons? I
> am
> concerned about the amount of time this will take for a tech to babysit the
> stain and also about the consistency of the quality in the end result.?
> Your
> opinions are much appreciated.
>
>
>
> Thank you!
>
>
>
> Jennifer
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 9 Feb 2017 16:19:00 +0000
> From: Walter Benton <wbenton at cua.md>
> To: Rene J Buesa <rjbuesa at yahoo.com>, Jennifer
>         <histotech at dermspec.com>
> Cc: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] hand staining immunos
> Message-ID: <0952dbba122f4678aa83759b2c98d994 at MAIL01.GCU-MD.local>
> Content-Type: text/plain; charset="utf-8"
>
> http://www.agilent.com/cs/library/technicaloverviews/
> public/08002_ihc_staining_methods.pdf
>
>
> -----Original Message-----
> From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Thursday, February 09, 2017 10:51 AM
> To: Jennifer <histotech at dermspec.com>; histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] hand staining immunos
>
> You do not have to "babysit" the procedure for it has well defined/timed
> steps. You just need a timer and check the slides when required. As to
> procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren?
>
>     On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
>
>  Hello,
>
>
>
> My dermatopathologist wants me to start hand staining immunos  for MITF. I
> would really like some feedback about everyone's experiences/thoughts with
> manually hand staining immunos and especially with MITF. Pros and cons? I
> am concerned about the amount of time this will take for a tech to babysit
> the stain and also about the consistency of the quality in the end result.
> Your opinions are much appreciated.
>
>
>
> Thank you!
>
>
>
> Jennifer
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
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> ------------------------------
>
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