[Histonet] hand staining immunos

[Walter Benton]

http://www.agilent.com/cs/library/technicaloverviews/public/08002_ihc_staining_methods.pdf


-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, February 09, 2017 10:51 AM
To: Jennifer <histotech at dermspec.com>; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] hand staining immunos

You do not have to "babysit" the procedure for it has well defined/timed steps. You just need a timer and check the slides when required. As to procedural "dos & donts" try to get a copy of the DAKO IHC manual.René

    On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet <histonet at lists.utsouthwestern.edu> wrote:


 Hello,



My dermatopathologist wants me to start hand staining immunos  for MITF. I would really like some feedback about everyone's experiences/thoughts with manually hand staining immunos and especially with MITF. Pros and cons? I am concerned about the amount of time this will take for a tech to babysit the stain and also about the consistency of the quality in the end result.  Your opinions are much appreciated.



Thank you!



Jennifer

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