[Histonet] Eosin B solution for H&E

Bryan Llewellyn llewllew at shaw.ca
Sun Feb 5 13:04:22 CST 2017

I played around with it many years ago and found no difference except 
for the bluer tone. Simply substitute it for eosin Y ws gram for gram, 
and use the same technique.

Bryan Llewellyn

Bob Richmond via Histonet wrote:
> Gudrun Lang (in Austria) asks:
>>> I wonder how widespread the usage of eosin B is in H&E protocols. Is
> there a
> specific difference in application to Eosin Y?<<
> Eosin B is closely related to the more commonly used eosin Y. As the
> letters imply, it's Bluish rather than Yellowish.
> I've never seen eosin B used in H&E staining protocols, but it certainly
> has been.
> Bob Richmond
> Samurai Pathologist
> Maryville, Tennessee
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