[Histonet] Modified GMS Protocols
nipuna.we at gmail.com
Sun Dec 31 06:56:23 CST 2017
Thanks for your reply Gudrun,
I agree with your statements. If KMnO4 used as a replacement for CrO3, it
should be used under much milder conditions (low temperature and less
incubation time) than H6IO5 as KMnO4 is a stronger oxidant. However, my
question was due to the fact that KMnO4 was a commonly used oxidant and
often compared with both CrO3 and H6IO5. Lillie and McManus showed that all
three can be used to stain same carbohydrate targets (Modified PAS).
Even some of the recent papers discuss about GMS based on KMnO4 oxidation
but I cannot see protocols, on-market kits or papers actually reporting
any. If KMnO4 can work in PAS why it is not tried in GMS? So I thought
histologists work in the filed have some experience.
Lillie, R. D. Histochemical Comparison of the Casella, Bauer, and
Periodic Acid Oxidation-Schiff Leucofuchsin Technics. *Stain. Technol.*
1951, *26*, 123–136.
McManus, J. F. A. Periodate Oxidation Techniques. In General
Cytochemical Methods; Academy Press Inc., 1961; Vol. 2
Speranza, V. D.; Fail, R. A Common Mistake When Staining for Fungi.
Histol. Tech. Bull. Histotechnol. 2004.
On Sat, Dec 30, 2017 at 1:44 AM, Gudrun Lang <gu.lang at gmx.at> wrote:
> Biological stains were mainly found by trial and error through the
> century. I think the main goals were to find a good result (for the
> purpose) within the available resources (cheap / expensive, easy/difficult
> to get) and with a practical application.
> Later also health-concerns played a role, that eliminated some protocols.
> I believe, that the "forefathers" had a hard time to find the best-working
> protocols. And after that, the histological community acts upon the
> sentence "never change a winning team" and sticks to the protocols. And we
> have to admit, that the chemical knowledge of the histologists and the
> histological knowledge of the chemists may be rather decreased than grown
> bigger. (a lack of universal scientists)
> For the diverse oxidants I think (without literature as evidence) it is
> also a practical matter. The oxidizising result depends on strength of acid
> and duration of incubation. If you use a very strong oxidant, the time has
> to be watched very carefully and there is the risk of overoxidation. If you
> use a weaker oxidant, you have a longer time-space for a sufficient result.
> (5 min in periodic acid may refer to 20 sek in potassiumpermanganat ?)
> -----Ursprüngliche Nachricht-----
> Von: Nipuna Weerasinghe via Histonet [mailto:histonet at lists.
> Gesendet: Donnerstag, 28. Dezember 2017 20:30
> An: histonet at lists.utsouthwestern.edu
> Betreff: [Histonet] Modified GMS Protocols
> I would like to know from subject matter expert why KMno4 never used as an
> alternative oxidant to CrO3 in Modified GMS. Oxidation of 1,2-glycol
> linkages in carbohydrates to aldehyde groups can be done by KMNO4 and used
> to do the same in Castella’s potassium permanganate-Schiff reaction, and
> Gordon and Sweets' reticulum. Moreover, KMNO4 is a strong oxidant that can
> oxidized aldehyde to carboxylic, so this leads to closer mimicking of
> function of CrO3.
> Also why people only tried periodic and not any other oxidant to replace
> CrO3. I could not find any primary literates concnering this matter. Only
> handful of attempts with periodic is there.
> Thanks for your answers in advanced
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