[Histonet] Mouse bladder

[Colleen Forster]

Exactly...and then it wont shrivel up....

C

On Tue, Dec 19, 2017 at 3:09 PM, dusko trajkovic <dunatrsd at sbcglobal.net>
wrote:

> I agree with Colleen, processing is the issue. You need to process on a
> longer program in order to dehydrate and clear the agar properly.
> dusko
>
>
> On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
>
> Be sure you are processing them on an overnight process. Even if the
> samples are small it takes that time to process the agar properly.
>
> I use Histogel and learned this lesson the hard way. My samples always
> "shriveled" up too until someone enlightened me about the processing time.
> I would think agar might be the same.
>
>
> Just a thought,
>
> Colleen Forster HT(ASCP)QIHC
> U of MN
>
> On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
> > I would try histogel instead of agar.
> >
> > Liz
> >
> > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> > Premier Laboratory, LLC
> > PO Box 18592
> > Boulder, CO 80308
> > (303) 682-3949 office
> > (303) 682-9060 fax
> > (303) 881-0763 cell
> > liz at premierlab.com<mailto:liz at premierlab.com>
> > www.premierlab.com<http://www.premierlab.com/>
> >
> > Ship to Address:
> >
> > Premier Laboratory, LLC
> > 1567 Skyway Drive, Unit E
> <https://maps.google.com/?q=1567+Skyway+Drive,+Unit+E+%3E+Longmont,+CO+80504&entry=gmail&source=g>
> > Longmont, CO 80504
> <https://maps.google.com/?q=1567+Skyway+Drive,+Unit+E+%3E+Longmont,+CO+80504&entry=gmail&source=g>
> >
> > From: Heather Marlatt via Histonet [mailto:histonet at lists.
> > utsouthwestern.edu]
> > Sent: Tuesday, December 19, 2017 9:12 AM
> > To: histonet at lists.utsouthwestern.edu
> > Subject: [Histonet] Mouse bladder
> >
> > Does anyone have a good protocol for mouse bladder embedded in agar that
> > they are willing to share? We have mouse bladders in 2% agar and have
> tried
> > a few different protocol variations from our usual mouse but the agar
> keeps
> > shriveling up.
> >
> > Thanks
> > Heather
> > _______________________________________________
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.
> utsouthwestern.edu
> > >
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> >
> > ________________________________
> > _______________________________________________
> > Histonet mailing list
> > Histonet at lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>