[Histonet] Mouse bladder

[dusko trajkovic]

I agree with Colleen, processing is the issue. You need to process on a longer program in order to dehydrate and clear the agar properly.dusko 

    On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 

 Be sure you are processing them on an overnight process. Even if the
samples are small it takes that time to process the agar properly.

I use Histogel and learned this lesson the hard way. My samples always
"shriveled" up too until someone enlightened me about the processing time.
I would think agar might be the same.


Just a thought,

Colleen Forster HT(ASCP)QIHC
U of MN

On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> I would try histogel instead of agar.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> liz at premierlab.com<mailto:liz at premierlab.com>
> www.premierlab.com<http://www.premierlab.com/>
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> From: Heather Marlatt via Histonet [mailto:histonet at lists.
> utsouthwestern.edu]
> Sent: Tuesday, December 19, 2017 9:12 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Mouse bladder
>
> Does anyone have a good protocol for mouse bladder embedded in agar that
> they are willing to share? We have mouse bladders in 2% agar and have tried
> a few different protocol variations from our usual mouse but the agar keeps
> shriveling up.
>
> Thanks
> Heather
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