[Histonet] Histonet Digest, Vol 169, Issue 3

Tue Dec 5 12:41:07 CST 2017

Tendons - use a tungsten carbide blade/ 50 degree.  

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Sent: Tuesday, December 5, 2017 12:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 169, Issue 3

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Today's Topics:

   1. ANP.10255 (Adesuyi, Banjo)
   2. Science Haiku (Amos Brooks)
   3. looking for diff cell counter (Lauren Sweeney)
   4. Noise Levels in Lab (Melissa Burns)
   5. Microtomy of Equine Tendon Blocks (Alan)
   6. Re: Noise Levels in Lab (Morken, Timothy)

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Message: 1
Date: Mon, 4 Dec 2017 19:14:53 +0000
From: "Adesuyi, Banjo" <abadesuyi at nrh-ok.com>
To: "'histonet at lists.utsouthwestern.edu'"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] ANP.10255
Message-ID: <4ea201e15dce48ef808fec40982ea340 at nrh-ok.com>
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Good afternoon,
                            Please I am wondering whether you can direct me to where I can get a policy for the pathologist's participation in a peer educational program and internal audits

Best regards,
Adesupo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS, Supervisor, Histology Section abadesuyi at nrh-ok.com<mailto:abadesuyi at nrh-ok.com>
O: 405-307-1145
M: 405-973-6363
F:  405-307-1143

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Message: 2
Date: Mon, 4 Dec 2017 20:08:59 -0500
From: Amos Brooks <amosbrooks at gmail.com>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Science Haiku
Message-ID:
	<CAC95ki_ea6c0VP4AkM_+W+qHXGRZg=a36VVbhm2rFwfVC1kYdg at mail.gmail.com>
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Anyone that heard Science Friday recently may have heard of this. I thought it would be fun to see what others come up with. So many people think I'm an archaeologist when I tell them I'm a Histotech. This makes for a fun elevator pitch for our profession.

Scientists are challenged to summarize their work in a haiku. Here's mine...

Sick organs need tests
It's microscopic study
Thats histology

#flamechallenge
<https://www.facebook.com/hashtag/flamechallenge?source=feed_text&story_id=1735098133176169>

http://www.stonybrook.edu/happenings/community-outreach/35567/

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Message: 3
Date: Tue, 5 Dec 2017 13:32:39 +0000
From: Lauren Sweeney <lmarie08 at uga.edu>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] looking for diff cell counter
Message-ID:
	<BN6PR02MB31376050B1BFED1F30BFC498C53D0 at BN6PR02MB3137.namprd02.prod.outlook.com>

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Good Morning Histonet colleagues,

My PI wants me to purchase a differential cell counter and I was wondering if anyone had any recommendations for a basic manual counter. One that offers the ability to count 5 different cell types and dings at every 100 cells. I found one through Fisher but it seems pricey. Does anyone know other places to get them?

Thanks!

Lauren

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Message: 4
Date: Tue, 5 Dec 2017 13:45:40 +0000
From: Melissa Burns <mburns at atlanticurologyclinics.com>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Noise Levels in Lab
Message-ID:
	<8C537C608EA0194B87F6DE7BC6E1BF885BF11CBF at AUC-Exchange2.gsuro.com>
Content-Type: text/plain; charset="us-ascii"

Does anyone monitor the noise levels in their lab? If so, what is your protocol? Do you have a  device to do this?

Thanks in advance :)

Melissa

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Message: 5
Date: Tue, 5 Dec 2017 16:03:07 +0000
From: Alan <aj.taylor at blueyonder.co.uk>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Microtomy of Equine Tendon Blocks
Message-ID:
	<mailman.2.1512496801.26087.histonet at lists.utsouthwestern.edu>
Content-Type: text/plain; charset="utf-8"

Greetings to all on Histonet. I have a particularly challenging question regarding the sectioning of Equine tendon material. My colleague and I recently received a number of TS tendon slices. Unfortunately they had been placed in 90% IDA for fixation approximately 10 years ago, where they remained up to the point they were sent to our lab for cutting and staining. 
The blocks are extremely hard, almost like undecalcified bone. They have processed well into paraffin, but we have wrecked several disposable blades just in facing the blocks. They have a very gritty feel on trimming even at 2 microns.
We have tried some commercial softening agents, including Mollifex, with long immersion over days, rather than the usual hour or so, we have tried neat fabric softener and 10% phenol in 70% IDA. We have also left some blocks in molten paraffin for several days, as this sometimes works with very fibrous tissues. As does prolonged chilling with ice and water.
We have managed to recover a small number of reasonable sections, at present we are looking at 1 block = 1 blade to obtain a very few working sections.
Do any histotechs out there have any secret recipes that they are prepared to share and have made up themselves, or a popular in-house reagent that they know is reliable,  that they have enjoyed success  with in their labs, or references to softening solutions for very difficult tissues, that may be historical or more recent. We have, of course, asked the referring lab to place all future freshly dissected tissues immediately into 10%NBF. Thank you for any assistance you are able to offer.

Yours sincerely
Alan Taylor BSc(Hons). FRMS
Microtechnical Services
Exeter. UK
www.microtechnicalservices.co.uk 

Sent from Mail for Windows 10

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Message: 6
Date: Tue, 5 Dec 2017 16:01:59 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: Melissa Burns <mburns at atlanticurologyclinics.com>
Cc: Histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Noise Levels in Lab
Message-ID:
	<CY1PR0501MB127520606730CC32414454E7E73D0 at CY1PR0501MB1275.namprd05.prod.outlook.com>

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We don't but there are decibel monitor phone apps. Not sure how accurate they are...

I've used one called Sound Meter. It shows 70+ db next to our -80 freezer and 90+ db next to the 6-foot fume hood.

In our lab with cryostats it shows 60+ and in a quiet lab, no equipment running, it shows 50+

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

-----Original Message-----
From: Melissa Burns via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, December 05, 2017 5:46 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Noise Levels in Lab

Does anyone monitor the noise levels in their lab? If so, what is your protocol? Do you have a  device to do this?

Thanks in advance :)

Melissa
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End of Histonet Digest, Vol 169, Issue 3
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