[Histonet] decal protocol with EDTA for bone marrow biopsies

[Пешков Максим]

We fixed our BM biopsies at least 24 hours, but no more than 72 h in 10% NBF.
Wash with tap water 30 mins.
Decal into 14% EDTA (2Na salt) buffered with NaOH up to 7.0 overnight or weekend and test endpoint by weight loss weight gain method (special thanks to Gayle Callis!).
Wash in tap water 1 hour.
Process with isopropanols (IPA), mixtures IPA and mineral oil), paraffin wax.
Microtomy at 3 microns.
Stain HE, Giemsa, HC, IHC.
--
Maxim Peshkov,
Russia,
Taganrog.